We narrowed to 5,358 results for: PID;

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siRNA-resistant ABHD16A-Halo

Plasmid

#195163

Purpose

mammalian expression of siRNA-resistant ABHD16A tagged with Halo tag

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Tags

Halo tag

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TMEM16A-I546K-peGFP-N1

Plasmid

#228582

Purpose

Expresses mouse TMEM16A-I546K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)

Expression

Mammalian

Mutation

I546K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AD101

Plasmid

#131402

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody AD101

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Golgin-84-TEV #709#243

Plasmid

#42108

Depositor

Ayano Satoh

Article

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Insert

Golgin-84 (GOLGA5 Human)

Tags

GFP

Expression

Mammalian

Mutation

inserted ENLYFQG b/w 300 and 301

Promoter

CMV

Available Since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
pRDA_936

Plasmid

#216094

Purpose

Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD46, CD47, CD55, CD81 guides

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11A(E186Q)-HA

Plasmid

#209249

Purpose

Mammalian expression of ATP11A mutant E186Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11A (ATP11A Human)

Tags

HA

Expression

Mammalian

Mutation

E186Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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CoV2-N-T205I

Plasmid

#177954

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (T205I) (N )

Expression

Mammalian

Mutation

T205I

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1-HLx2

Plasmid

#108133

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]2:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]2:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRDB_181

Plasmid

#216102

Purpose

CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP2 D61A

Plasmid

#8320

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Mutation

D61A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
AA331

Plasmid

#215954

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mCherry-mTrim21ΔRING-Box

Plasmid

#105523

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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YF-Cdc42(CA)

Plasmid

#20152

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Cdc42(CA) (CDC42 Human)

Expression

Mammalian

Mutation

Cdc42H was inserted into the multiple cloning sit…

Available Since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

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pRDA_889

Plasmid

#216080

Purpose

ITRs from AAV2, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

ITRs from AAV2

Use

AAV; Destination

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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CoV2-N-R203K

Plasmid

#177951

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (R203K) (N )

Expression

Mammalian

Mutation

R203K

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJPT018-LAT_WT-HaloTag-3x FLAG

Plasmid

#202502

Purpose

Express WT-LAT

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

LAT (LAT Human)

Tags

HaloTag-3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP

Plasmid

#199367

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-SUMOEu-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMK285 (Stag-3FLAG-Bsr)

Plasmid

#72801

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Bsr

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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Pvha6-AgDD

Plasmid

#80626

Purpose

create protein aggregates in C. elegans

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NES-L106P-sfGFP (FKBP1A Human)

Tags

MLALKLAGLDI

Expression

Worm

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 PuroR L3-L2

Plasmid

#62164

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Puromycin

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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