We narrowed to 3,807 results for: golden gate

1. pYPQ134-STU-Fn

   Plasmid

   #138104

   Purpose

   Golden Gate entry vector for 4th crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYPQ131-STU-Fn

   Plasmid

   #138095

   Purpose

   Golden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ132-STU-Fn

   Plasmid

   #138098

   Purpose

   Golden Gate entry vector for 2nd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ133-STU-Fn

   Plasmid

   #138101

   Purpose

   Golden Gate entry vector for 3rd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTW334e2b

   Plasmid

   #246587

   Purpose

   sequential knock out of A. thaliana Rubisco assembly factors

   Depositor

   Tina Wang

   Article

   Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

   Insert

   AtRaf1, AtRaf2, AtRbcX2, AtBSD2W108A/L109E, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)
   

   Expression

   Bacterial

   Mutation

   AtBSD2W108A/L109E

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTW334e4

   Plasmid

   #246589

   Purpose

   sequential knock out of A. thaliana Rubisco assembly factors

   Depositor

   Tina Wang

   Article

   Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

   Insert

   AtRaf1, AtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN20, RbcX2, CPN60B, CPN60A Mustard Weed)
   

   Expression

   Bacterial

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTW334e2

   Plasmid

   #246586

   Purpose

   sequential knock out of A. thaliana Rubisco assembly factors

   Depositor

   Tina Wang

   Article

   Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

   Insert

   AtRaf1, AtRaf2, AtRbcX2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)
   

   Expression

   Bacterial

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. L4385 dsRedExpress2 reporter, IL-10Rb NTEVp, IL-10Ra CTEVp in PiggyBac Transposon Vector

   Plasmid

   #244186

   Purpose

   PiggyBac transposon vector for expression of dsRedExpress2 under synTF promoter; constitutive expression of IL-10Rb NTEVp chain, IL-10Ra CTEVp chain, and mNeonGreen-P2A-PuroR selection marker

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Insert

   dsRedExpress2 under synTF responsive promoter; IL-10Rb NTEVp chain with human CD8a SS; IL-10Ra CTEVp chain; mNeonGreen-P2A-PuroR (CD8A Human, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   NTEVp_75S, CTEVp_190K

   Promoter

   hEF1a

   Available Since

   Oct. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-U6-sgNT-2-EF1a-LibVec

   Plasmid

   #239593

   Purpose

   expresses non-targeting (NT) guide#2

   Depositor

   Andreas Moor

   Article

   Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

   Insert

   NT (non-targeting guide)
   

   Use

   AAV; To deliver a non-targeting guide (serving as…

   Promoter

   hU6

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-U6-sgNT-1-EF1a-LibVec

    Plasmid

    #239592

    Purpose

    expresses non-targeting (NT) guide#1

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    NT (non-targeting guide)
    

    Use

    AAV; To deliver a non-targeting guide (serving as…

    Promoter

    hU6

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNK5664

    Plasmid

    #219747

    Purpose

    DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pCMV - nnLuz_v4 - tSV40
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNK291

    Plasmid

    #219748

    Purpose

    DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of CMV promoter, for mammalian expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pCMV - nnH3H_v2 - tSV40
    

    Expression

    Mammalian

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLCHKOv3-mClover-intergenic_2-As

    Plasmid

    #218814

    Purpose

    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLCHKOv3-mClover-intergenic_3-As

    Plasmid

    #218815

    Purpose

    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLCHKOv3-mClover-intergenic_4-As

    Plasmid

    #218816

    Purpose

    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218915

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-MUTT2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
    

    Expression

    Mammalian

    Mutation

    2363G>C and 4330T>C

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218913

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-MUTT2A-SARS-CoV2-NCAP/BlastR (N )
    

    Expression

    Mammalian

    Mutation

    2363G>C and 5335T>C

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218912

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-SARS-CoV2-NCAP/BlastR (N )
    

    Expression

    Mammalian

    Mutation

    2363G>C and 5335T>C

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB-TRE-V5-TurboID-linker-MUTT2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218911

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-MUTT2A-huGR/BlastR (NR3C1 Human)
    

    Expression

    Mammalian

    Mutation

    2363G>C and 4155C>T and 4729T>G and 4731…

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ132-STU-As

    Plasmid

    #138097

    Purpose

    Golden Gate entry vector for 2nd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge