We narrowed to 4,995 results for: mcs

1. pLKO-perox-mEm

   Plasmid

   #185597

   Purpose

   Lentiviral construct for expression of mEmerald targeted to peroxisomes

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   perox-mEm
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-AcGFP-progerin

   Plasmid

   #86947

   Purpose

   Construct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.

   Depositor

   Alex Hewitt

   Insert

   AcGFP-progerin (LMNA Human)
   

   Use

   AAV

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   progerin sequence is LMNA gene with nucleotide C1…

   Promoter

   CMV and T7

   Available Since

   June 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSII42H-TDH3pr

   Plasmid

   #194533

   Purpose

   High copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

   Depositor

   Patrick Gibney

   Article

   Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVITRO1-TagRFP-NLS

   Plasmid

   #184446

   Purpose

   Nucleus-targeted TagRFP expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   TagRFP-3xNLS
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKHR7

   Plasmid

   #74624

   Purpose

   a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.

   Depositor

   David Grunwald

   Article

   Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

   Insert

   cmlc2::mCherry
   

   Use

   Zebrafish expression

   Promoter

   cmlc2

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-cell-mEm

   Plasmid

   #185595

   Purpose

   Lentiviral construct for expression of mEmerald

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   cell-mEm
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL3-TRE-LucGFP-2L

   Plasmid

   #11685

   Depositor

   Geoff Wahl

   Article

   Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

   Insert

   Luciferase-EGFP
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVITRO1-mCherry-NES

   Plasmid

   #186349

   Purpose

   Cytosolic mCherry expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   mCherry-NES
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVITRO1-SS-338

   Plasmid

   #68795

   Purpose

   Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   C-Terminal Sensor 1 V1
   mCherry
   ZF9 Op x6
   EGFP
   N-Terminal Sensor 1 V2
   

   Tags

   IRES, NLS, flag, and nls

   Expression

   Mammalian

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVITRO1-BFP-NLS

    Plasmid

    #184458

    Purpose

    Nucleus-targeted EBFP2 expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    EBFP2-3xNLS
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVITRO1-BFP-LaminB1

    Plasmid

    #184460

    Purpose

    Nuclear membrane-targeted EBFP2 expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    EBFP2-LaminB1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-2xMARS-nGFP-puro

    Plasmid

    #205238

    Purpose

    Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Lentiviral

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRVdG-mCherry-N-P-M-EGFP-SynPhRFP-L

    Plasmid

    #52481

    Purpose

    Expresses mCherry, EGFP and Synaptophysin-TagRFP-T

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

    Inserts

    mCherry
    EGFP
    Synaptophysin-TagRFP-T
    

    Use

    Rabies virus

    Tags

    TagRFP-T

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHIE041 CAG mKate2 (TUPV 1)

    Plasmid

    #138721

    Purpose

    mMoClo TUPV, with CAG promoter and mKate2 in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    CAG
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO-mito-mCer

    Plasmid

    #185602

    Purpose

    Lentiviral construct for expression of mCerulean targeted to mitochondria

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    mito-mCer
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

    Plasmid

    #203911

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C2]-VP64
    

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

    Plasmid

    #203910

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C1]-VP64
    

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVITRO1-iRFP702-LaminB1

    Plasmid

    #184456

    Purpose

    Nuclear membrane-targeted iRFP702 expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    iRFP702-LaminB1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pShuttle-CMV-EGFP-C

    Plasmid

    #13887

    Depositor

    Lorene Lanier

    Article

    Strasser et al Neuron. 2004 Jul 8. 43(1):81-94.

    Insert

    C domain of N-WASP (WASL Bovine)
    

    Use

    Adenoviral

    Tags

    EGFP

    Mutation

    The BglII site in the MCS of pShuttle-CMV was fil…

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVITRO1-TagRFP-NES

    Plasmid

    #184449

    Purpose

    Cytosolic TagRFP expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    TagRFP-NES
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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