We narrowed to 5,650 results for: pCas

1. pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)

   Plasmid

   #160631

   Purpose

   Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.

   Depositor

   Diego Orzaez

   Insert

   gRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U626

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

   Plasmid

   #160640

   Purpose

   Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

   Depositor

   Diego Orzaez

   Insert

   Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos, 35S

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CJ30 (miR-1003 minigene)

   Plasmid

   #17767

   Depositor

   David Bartel

   Article

   Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

   Insert

   dme-miR-1003 minigene
   

   Use

   Drosophila p-element vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pCX138

   Plasmid

   #229213

   Purpose

   CRISPR array plasmid for NOTCH2 (Spacers 25-48)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for NOTCH2 (Spacers 25-48)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330.puro

   Plasmid

   #110403

   Purpose

   CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

   Depositor

   Sandra Martha Gomes Dias

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABEmax-SpRY

   Plasmid

   #195279

   Purpose

   Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

   Depositor

   Petros Patsali, Carsten Lederer

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PBCAG-eGFP

   Plasmid

   #40973

   Depositor

   Joseph Loturco

   Article

   Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

   Inserts

   PiggyBac transposon 3' terminal repeats
   PiggyBac 5' terminal repeats
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRho-DsRed

   Plasmid

   #11156

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Insert

   rhodopsin promoter (RHO Bovine)
   

   Use

   Tags

   DsRed2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. ovoD1 D1B2NR (18 kb)

   Plasmid

   #37280

   Depositor

   Norbert Perrimon

   Article

   Chou et al Development. 1993 Dec;119(4):1359-69.

   Insert

   ovoD1 genomic DNA fragment
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pTALYM4

    Plasmid

    #47875

    Purpose

    Destination plasmid with mRuby2 for Golden Gate reaction of TALE assembly

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Type

    Empty backbone

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABEmax-SpG

    Plasmid

    #195278

    Purpose

    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP

    Depositor

    Petros Patsali, Carsten Lederer

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCVA-amiR-PDS

    Plasmid

    #32911

    Depositor

    Yule Liu

    Article

    Tang et al Plant Physiol. 2010 Jun;153(2):632-41. E

    Insert

    amiR-PDS (Nb)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTALYM9

    Plasmid

    #47876

    Purpose

    Destination plasmid with 3xTy1 tags for Golden Gate reaction of TALE assembly

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Ty1tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. HiFi iFE

    Plasmid

    #190141

    Purpose

    Expresses HiFiCas9-POLB (HiFi iFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    HiFi Cas9-POLB
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    SpCas9 R691A mutant, Codon Optimized for mammalia…

    Promoter

    CBh

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid

    #160647

    Purpose

    module containing the human codon optimized Cas9 and the BASTA selection marker

    Depositor

    Diego Orzaez

    Insert

    BASTA / Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, P35S

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSI-356 v2

    Plasmid

    #158431

    Purpose

    SpCas9 sgRNA cloning backbone

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Methods Mol Biol. 2021;2312:193-223. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human H6

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX379 Legionella pneumophila str. Paris Cas9

    Plasmid

    #68316

    Purpose

    Legionella pneumophila str. Paris Cas9

    Depositor

    Feng Zhang

    Insert

    LpCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. ovoD1 D1B2H (7.2 kb)

    Plasmid

    #37282

    Depositor

    Norbert Perrimon

    Article

    Chou et al Development. 1993 Dec;119(4):1359-69.

    Insert

    ovoD1 genomic DNA fragment
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

19. ovoD1 D1B2R#225 (10 kb)

    Plasmid

    #37281

    Depositor

    Norbert Perrimon

    Article

    Chou et al Development. 1993 Dec;119(4):1359-69.

    Insert

    ovoD1 genomic DNA fragment
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pX330-MM002-ZFP143-C-terminal-sgRNA

    Plasmid

    #212706

    Purpose

    Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus

    Depositor

    Elzo de Wit

    Insert

    ZFP143 C-terminal sgRNA (Zfp143 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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