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Showing: 1851 - 1860 of 169284 results

  2. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87844-plasmid-map-sequence-id-174620

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  6. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100903-plasmid-map-sequence-id-196607

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  7. qTAG-C-Solo-mStayGold-TOMM20

    Plasmid
    #227307
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227307-plasmid-map-sequence-id-451546

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  10. pHeACT-mEGFP-NLS

    Plasmid
    #205003
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
    Use
    Tardigrade expression
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    205003-plasmid-map-sequence-id-405176

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Showing: 1851 - 1860 of 169284 results