We narrowed to 34,848 results for: CaS;

1. pCasper4-g-osk-TRICK

   Plasmid

   #71244

   Purpose

   oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   oskar (osk Fly)
   

   Use

   P element-mediated germline transformation

   Tags

   12xPP7 and 6xMS2

   Expression

   Insect

   Mutation

   Promoter

   osk

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAWPSG-Ptet-nCas9-BE

   Plasmid

   #195739

   Purpose

   Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet(Tetracycline inducible protein)

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21b-Caspase-3(C163S)

   Plasmid

   #90088

   Purpose

   Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   C163S

   Promoter

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. plenti-Lb-Cas12a-2xNLS

   Plasmid

   #155046

   Purpose

   Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS, Myc-tag and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-spCas9-pNeo

   Plasmid

   #214120

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Promoter

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330a dCas9-VP64

   Plasmid

   #92363

   Purpose

   Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAaePUb::Cas9-2A-Neo

   Plasmid

   #176680

   Purpose

   Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Insert

   spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
   

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Drosophila codon optimized

   Promoter

   Ae. aegypti poly-ubiquitin (AAEL003888)

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Cas9-162A

   Plasmid

   #84918

   Purpose

   for in vitro transcription of Cas9 with polyA tail encoded

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Cas9
   

   Use

   Tags

   Flag, NLS, and polyA tail

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. RCASBP(A)-3xFlag-mHoxd13wt

   Plasmid

   #145288

   Purpose

   Avian Expression of murine wildtype Hoxd13 with N-terminal 3xFlag tag

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   Hoxd13(wt) (Hoxd13 Mouse)
   

   Use

   Viral avian expression

   Tags

   3xFlag

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459V2.0-SpCas9-HF1

    Plasmid

    #108293

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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