Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 116,152 results for: mpi

Showing: 9251 - 9300 of 116152 results

  1. pMX-Flag-GFP_TMEM256

    Plasmid
    #227955
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    TMEM256 (C17orf61 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227955-plasmid-map-sequence-id-455415

    Enlarge

  2. pMX-Flag-GFP_CBLN3

    Plasmid
    #227950
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    CBLN3 (CBLN3 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227950-plasmid-map-sequence-id-455399

    Enlarge

  3. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

    Enlarge

  13. pHR-TRE-miRFP703_PGK-Puro

    Plasmid
    #216667
    Purpose
    Expresses tTa-inducible miRFP703, with puromycin resistance
    Depositor
    Leonardo Morsut
    Inserts
    miRFP703
    PuroR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216667-plasmid-map-sequence-id-432197

    Enlarge

  19. CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268

    Plasmid
    #227089
    Purpose
    Bacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)
    Depositor
    Chris Hill
    Insert
    tom1p-HECT (TOM1 Budding Yeast)
    Use
    Tags
    HIS-MBP-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227089-plasmid-map-sequence-id-451122

    Enlarge

  20. CPH3566_GST-TEV-Spt6p-tSH2-1223-1451

    Plasmid
    #227085
    Purpose
    Bacterial expression of GST-tagged Spt6 tSH2 domain (1223-1451)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Use
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227085-plasmid-map-sequence-id-451055

    Enlarge

  21. pRS415 GPDpro-RNQ1-CFP

    Plasmid
    #224887
    Purpose
    Low copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).
    Depositor
    Paul Herman
    Insert
    RNQ1 (RNQ1 Budding Yeast)
    Use
    Tags
    CFP
    Expression
    Yeast
    Mutation
    Promoter
    GPD
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224887-plasmid-map-sequence-id-448181

    Enlarge

  22. pAAV-phSyn-flex-SypHaloTag

    Plasmid
    #172280
    Purpose
    Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.
    Depositor
    Danny Winder
    Insert
    synaptophysin-HaloTag
    Use
    AAV and Cre/Lox
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    rat synapsin
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    172280-plasmid-map-sequence-id-337805

    Enlarge

  23. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Katherine Walstrom
    Insert
    MDH-2 (mdh-2 Nematode)
    Use
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224146-plasmid-map-sequence-id-444697

    Enlarge

  24. pFastBac HTB His-TEV-BN-rNLRP1-FLAG

    Plasmid
    #217323
    Purpose
    Purification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217323-plasmid-map-sequence-id-438442

    Enlarge

  25. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

    Plasmid
    #217320
    Purpose
    Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217320-plasmid-map-sequence-id-438445

    Enlarge

  26. H2B-Electra2

    Plasmid
    #218929
    Purpose
    Electra2 fused to human H2B protein for nuclear localization
    Depositor
    Amro Hamdoun
    Insert
    H2B-Electra2 (H2B Human, Entacmaea quadricolor, Synthetic)
    Use
    Synthetic Biology
    Tags
    Electra2 and H2B
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218929-plasmid-map-sequence-id-433882

    Enlarge

  27. LvPolyUb

    Plasmid
    #218982
    Purpose
    L.variegatus polyubiquitin-C promoter
    Depositor
    Amro Hamdoun
    Insert
    L.variegatus polyubiquitinC promoter
    Use
    Sea urchin
    Tags
    Expression
    Mutation
    Promoter
    L.variegatus polyubiquitinC promoter
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218982-plasmid-map-sequence-id-433881

    Enlarge

  28. Lmod1-BirA

    Plasmid
    #221509
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Lmod1
    Depositor
    Alessandro Ori
    Insert
    Lmod1 (Lmod1 Mouse)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221509-plasmid-map-sequence-id-439230

    Enlarge

  29. SYNJ1_BirA_Nterm

    Plasmid
    #221523
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Synj1
    Depositor
    Alessandro Ori
    Insert
    SYNJ1 (SYNJ1 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221523-plasmid-map-sequence-id-439225

    Enlarge

  30. SYNJ1_BirA_Cterm

    Plasmid
    #221522
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Synj1
    Depositor
    Alessandro Ori
    Insert
    SYNJ1 (SYNJ1 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221522-plasmid-map-sequence-id-439224

    Enlarge

  31. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

    Enlarge

  32. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

    Plasmid
    #217970
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.
    Depositor
    Robert Piper
    Insert
    2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    217970-plasmid-map-sequence-id-436105

    Enlarge

  33. MUT_S1-H1,NR

    Plasmid
    #218395
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218395-plasmid-map-sequence-id-433911

    Enlarge

  34. 1167A

    Plasmid
    #218233
    Purpose
    HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene
    Depositor
    Omar Akbari
    Insert
    putative metabolite transport protein
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    218233-plasmid-map-sequence-id-433264

    Enlarge

  35. pAG426-GAL-Prepro-3x-Beta-Amyloid

    Plasmid
    #181730
    Purpose
    Galactose inducible expression of Prepro-3x-Beta-Amyloid
    Depositor
    Alejandro Chavez
    Insert
    Prepro-3x-Beta-Amyloid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    181730-plasmid-map-sequence-id-431127

    Enlarge

  36. pAG426-GAL-Kar2-Beta-Amyloid

    Plasmid
    #181707
    Purpose
    Galactose inducible expression of Kar2-Beta-Amyloid
    Depositor
    Alejandro Chavez
    Insert
    Kar2-Beta-Amyloid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181707-plasmid-map-sequence-id-430950

    Enlarge

  37. SynCAM4/pCDNA3

    Plasmid
    #197328
    Purpose
    Plasmid expressing an antigen targeting NECL4/SynCAM4
    Depositor
    Matthew Rasband
    Insert
    SynCAM4 (Cadm4 Mouse)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    197328-plasmid-map-sequence-id-388225

    Enlarge

  38. pT7CFE1_polio_VP12A

    Plasmid
    #212988
    Purpose
    Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus type I Mahoney VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212988-plasmid-map-sequence-id-420361

    Enlarge

  39. pFastBac1-C17orf53

    Plasmid
    #211445
    Purpose
    generation of Bacmid for insect cell expression of C17orf53
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211445-plasmid-map-sequence-id-419422

    Enlarge

  40. pcDNA3-hFCER1G-VenusFlag

    Plasmid
    #211899
    Purpose
    Expresses human FCER1G in mammalian cells
    Depositor
    Haian Fu
    Insert
    FCER1G (FCER1G Human)
    Use
    Tags
    VenusFlag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    211899-plasmid-map-sequence-id-419135

    Enlarge

  41. pNHA_OROV_NSs

    Plasmid
    #208934
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 69 bp at 5' end (including ATG) and …
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208934-plasmid-map-sequence-id-418959

    Enlarge

  42. pLV-SFFV-mCherry-WPRE-UbC-Emerald

    Plasmid
    #211797
    Purpose
    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    mCherry
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211797-plasmid-map-sequence-id-418065

    Enlarge

  43. pPB-CAG-NPHP1

    Plasmid
    #209769
    Purpose
    Human NPHP1 (Nephrocystin 1) gene is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Yohei Hayashi
    Insert
    NPHP1 (NPHP1 Human, Synthetic)
    Use
    Piggybac transposon
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    209769-plasmid-map-sequence-id-417213

    Enlarge

  44. mEos Cav2.2 pRK5

    Plasmid
    #206091
    Purpose
    expression of rabbit Cav2.2 calcium channel with a N-terminal mEos tag
    Depositor
    Annette Dolphin
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    mEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    206091-plasmid-map-sequence-id-415595

    Enlarge

  45. mBeta4/pCNA3.1

    Plasmid
    #197316
    Purpose
    Plasmid expressing an antigen used to generate an antibody that targets the BK beta4 potassium channel subunit
    Depositor
    Robert Brenner
    Insert
    Slo B4 (Kcnmb4 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197316-plasmid-map-sequence-id-388157

    Enlarge

  46. CTHRC1A-c001

    Plasmid
    #205105
    Purpose
    CTHRC1 protein expression
    Depositor
    Paul Brennan
    Insert
    CTHRC1 (CTHRC1 Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205105-plasmid-map-sequence-id-412143

    Enlarge

  47. pMSCV-FLAG-puro-mOct4 S351A

    Plasmid
    #206913
    Purpose
    Expression of mOct4 S351A mutation
    Depositor
    Hyonchol Jang
    Insert
    Oct4 (Pou5f1 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    S351A
    Promoter
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    206913-plasmid-map-sequence-id-410080

    Enlarge

  48. GPC5A-c001

    Plasmid
    #205110
    Purpose
    GPC5 protein expression
    Depositor
    Paul Brennan
    Insert
    GPC5 (GPC5 Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    205110-plasmid-map-sequence-id-410361

    Enlarge

  49. pMSCV-FLAG-puro-mOct4 T87A

    Plasmid
    #206921
    Purpose
    Expression of mOct4 T87A mutation
    Depositor
    Hyonchol Jang
    Insert
    Oct4 (Pou5f1 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    T87A
    Promoter
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    206921-plasmid-map-sequence-id-410097

    Enlarge

  50. pMSCV-FLAG-puro-mOct4 S100A

    Plasmid
    #206920
    Purpose
    Expression of mOct4 S100A mutation
    Depositor
    Hyonchol Jang
    Insert
    Oct4 (Pou5f1 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    S100A
    Promoter
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    206920-plasmid-map-sequence-id-410096

    Enlarge

Showing: 9251 - 9300 of 116152 results