We narrowed to 10,984 results for: Vars;

1. pMig (Martins Lab)

   Plasmid

   #14125

   Depositor

   L. Miguel Martins

   Article

   Nicke et al Mol Cell. 2005 Dec 9. 20(5):673-85.

   Insert

   IRES-EGFP
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Cla I sites were silenced in the original pRetroS…

   Promoter

   Available since

   March 7, 2007

   Availability

   Academic Institutions and Nonprofits only

2. MS2_adRNA-MCP_ADAR2 DD_NLS

   Plasmid

   #124704

   Purpose

   Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NLS

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   MCP_ADAR2 DD_NLS
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GTH-y

   Plasmid

   #81105

   Purpose

   Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   mCitrine
   

   Use

   Cre/Lox

   Tags

   mCitrine

   Expression

   Yeast

   Mutation

   Mutated mCitrine for monomeric isoform (A206K L22…

   Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEY68

   Plasmid

   #191065

   Purpose

   ceh-14 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   ceh-14 (ceh-14 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHH0103_SPTAN1-1/1

   Plasmid

   #91292

   Purpose

   Protein expression and purification of human SH3 domain construct SPTAN1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SPTAN1-1/1 (SPTAN1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_SNX33-1/1

   Plasmid

   #91278

   Purpose

   Protein expression and purification of human SH3 domain construct SNX33-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SNX33-1/1 (SNX33 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA pDEST40 Cas9 GW STOP

   Plasmid

   #183196

   Purpose

   Destination vector

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti CRISPR V2 sgMTFMT_1

   Plasmid

   #106318

   Purpose

   Express Cas9 and sgRNA targeting MTFMT

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTFMT
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

   Plasmid

   #159873

   Purpose

   Three-fragment Gateway compatible protein tag for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmalc4x_I499F_TEV

    Plasmid

    #47703

    Purpose

    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

    Depositor

    Raquel Lieberman

    Article

    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

    Insert

    Olfactomedin-like domain with I499F mutation (MYOC Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed I499F

    Promoter

    Ptac

    Available since

    Sept. 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBAD-BirA-eCPX-AP(K10)

    Plasmid

    #121908

    Purpose

    Expresses BirA and eCPX-AP(K10)

    Depositor

    Per Svenningsen

    Article

    Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

    Inserts

    BirA (birA Synthetic)
    enhanced Circularly Permuted Outer Membrane Protein X
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 10 in the AP to alanine

    Promoter

    T7 and pBAD promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEY64

    Plasmid

    #191061

    Purpose

    aqp-6 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    aqp-6 (aqp-6 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

    Plasmid

    #192681

    Purpose

    Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSADdeltaG-GFP-AlstR

    Plasmid

    #32647

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Inserts

    GFP
    AlstR (AstA-R1 Fly)
    

    Use

    Rabies virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

15. tdEos-Vimentin-N-18

    Plasmid

    #57690

    Purpose

    Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    NM_003380.3 (VIM Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pECFP-Cdc42-Y40C

    Plasmid

    #105295

    Purpose

    Control for FRET biosensor analysis - cannot bind downstream effectors

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Y40C

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mSmo-NQ4-V5

    Plasmid

    #126416

    Purpose

    Localization, Expression of Smoothened NQ4 in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

    Insert

    m-Smoothened (Smo Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Smo glycosylation site NQ4

    Promoter

    Available since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_UBASH3A-1/1

    Plasmid

    #91478

    Purpose

    Protein expression and purification of human SH3 domain construct UBASH3A-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    UBASH3A-1/1 (UBASH3A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_NCK1-2/3

    Plasmid

    #91420

    Purpose

    Protein expression and purification of human SH3 domain construct NCK1-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCK1-2/3 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221 Cas9-HMGB1

    Plasmid

    #183198

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-HMGB1
    

    Use

    CRISPR; Gateway entry clone

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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