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p5E-dusp6

Plasmid

#82584

Purpose

5' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cells

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

FGF-responsive element of the dusp6 promoter

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pRho-DsRed

Plasmid

#11156

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

rhodopsin promoter (RHO Bovine)

Tags

DsRed2

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA3-C1-mCherry-LOVPLD

Plasmid

#219559

Purpose

ER-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

C1-mCherry-LOVPLD

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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GST-HA-GFP11-N1

Plasmid

#91953

Purpose

Expresses GST-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

GST-HA-GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-6xHis

Plasmid

#87343

Purpose

To express EGFP-6xHis in mammalian cell

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

6xHis

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDAS12069_U6-pegRNA-mCherry

Plasmid

#177180

Purpose

pegRNA cloning plasmid with mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMPRAduo:minP:GFP

Plasmid

#193739

Purpose

MPRAduo ORF (minP:GFP) donor

Depositor

Ryan Tewhey

Article

Mouri et al Cell Genom. 2022 Dec 16;3(1):100234. doi

Insert

GFP

Expression

Mammalian

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-b2AR-EYFP

Plasmid

#20948

Purpose

Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-b2AR

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAct-dCBEevoCDA1

Plasmid

#195515

Purpose

Ubiquitous expression of dCBEevoCDA1 in Drosophila

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

dCBE-evoCDA1

Use

CRISPR

Expression

Insect

Promoter

act5C

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

Plasmid

#214997

Purpose

Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

TMEM115 (Tmem115 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

UBC

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pT-CA-GFP-IRES-mLumin

Plasmid

#32749

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Inserts

CA-GFP
mLumin

Expression

Mammalian

Mutation

S158A and S65T

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCEP4-puroeGFP

Plasmid

#177922

Purpose

Constitutively expresses eGFP with puromycin selection

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-GFP1

Plasmid

#106408

Purpose

P-RAN-GFP1 (RANbody GFP1::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-GFP1

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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KBB464

Plasmid

#185097

Purpose

HA-tagged POM152 with glycosylation sites mutated and with GFP at C-terminus

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

Pom152DGlyc-GFP

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pNL[NLucP/IFNG/Hygro] Vector

Plasmid

#238626

Purpose

Express IFNG response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

IFNG (IFNG Human)

Use

Luciferase

Tags

NanoLuc (R)

Mutation

None

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET23a-mUbc9

Plasmid

#53137

Purpose

E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Ubc9 (Ube2i Mouse)

Tags

untagged

Expression

Bacterial

Mutation

protein sequence is identical to human

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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mARG1 cassette 1

Plasmid

#134343

Purpose

Mammalian acoustic reporter gene 1 cassette 1 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 1

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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mARG1 cassette 2

Plasmid

#134344

Purpose

Mammalian acoustic reporter gene 1 cassette 2 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAMPK alpha2 WT

Plasmid

#15991

Depositor

Morris Birnbaum

Article

Mu et al Mol Cell. 2001 May . 7(5):1085-94.

Insert

AMPK alpha2 (Prkaa2 Rat)

Tags

Myc

Expression

Mammalian

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pLV UBC APEX2-3xFLAG-IRES-eGFP

Plasmid

#214983

Purpose

Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

APEX2 (APX2 Glycine max (soybean))

Use

Lentiviral

Tags

3xFLAG

Promoter

UBC

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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