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We narrowed to 6,121 results for: cas9 expression plasmid

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Showing: 5581 - 5610 of 6121 results

  1. pUt-mNF-Cas13d

    Plasmid
    #224789
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-d2i
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224789-plasmid-map-sequence-id-445954

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  2. px552-sg-gria1-HT

    Plasmid
    #187652
    Purpose
    Contains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.
    Depositor
    Erin O'Shea
    Article
    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75
    Inserts
    HaloTag donor sequence
    miRFP670
    Use
    AAV and CRISPR
    Tags
    N/A
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    187652-plasmid-map-sequence-id-369120

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  3. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Article
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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  4. KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA

    Plasmid
    #121800
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121800-plasmid-map-sequence-id-237308

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  5. KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid
    #121804
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121804-plasmid-map-sequence-id-237313

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  6. KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

    Plasmid
    #121808
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121808-plasmid-map-sequence-id-237328

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  13. pGG438

    Plasmid
    #165486
    Purpose
    Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, S1136R, D1332K, and R1333G mutations in S…
    Promoter
    CMV and T7
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165486-plasmid-map-sequence-id-330963

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  15. pAID6

    Plasmid
    #136989
    Purpose
    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4
    Depositor
    Huimin Zhao
    Article
    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:
    Inserts
    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    Expression
    Yeast
    Promoter
    ENO2, TDH3, TEF1, and TPI1
    Available Since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    136989-plasmid-map-sequence-id-269844

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  16. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  17. pETM6-3F2-mCherry

    Plasmid
    #73418
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73418-plasmid-map-sequence-id-135318

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  18. pETM6-3A2-mCherry

    Plasmid
    #73425
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3A2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3A2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73425-plasmid-map-sequence-id-135325

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  19. pETM6-5F5-mCherry

    Plasmid
    #73422
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 5F5 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P5F5 (orthogonal T7-lac variant)
    Available Since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    73422-plasmid-map-sequence-id-135322

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  20. pETM6-1D4-mCherry

    Plasmid
    #73423
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1D4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1D4 (orthogonal T7-lac variant)
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73423-plasmid-map-sequence-id-135323

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  21. pETM6-4A6-mCherry

    Plasmid
    #73424
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4A6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4A6 (orthogonal T7-lac variant)
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73424-plasmid-map-sequence-id-135324

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  22. pETM6-1E4-mCherry

    Plasmid
    #73426
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1E4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1E4 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73426-plasmid-map-sequence-id-135326

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  23. pETM6-1B6-mCherry

    Plasmid
    #73420
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1B6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1B6 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73420-plasmid-map-sequence-id-135320

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  24. pETM6-3H5-mCherry

    Plasmid
    #73419
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3H5 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3H5 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73419-plasmid-map-sequence-id-135319

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  27. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

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Showing: 5581 - 5610 of 6121 results