We narrowed to 9,202 results for: sacs

1. pBF3060

   Plasmid

   #26853

   Purpose

   Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

   Depositor

   Nancy DaSilva, Suzanne Sandmeyer

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Insert

   CreA recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   GAL1

   Available since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-GFP(S65T)-kanMX6

   Plasmid

   #39292

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Aug. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEx-deletion-erm

   Plasmid

   #172213

   Purpose

   For allelic exchange in erythromycin-sensitive Prevotella copri strains

   Depositor

   Till Strowig

   Article

   Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

   Type

   Empty backbone

   Use

   Allelic exchange in prevotella copri

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA6a-13Myc-His3MX6

   Plasmid

   #41602

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   13Myc, A. gossypii translation elongation factor …

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQE-60 roGFP2-Orp1-His

   Plasmid

   #64976

   Purpose

   Bacterial expression of His-tagged roGFP2-Orp1

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Tags

   His and roGFP2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pME-QFGal4-SV40pA

   Plasmid

   #155118

   Purpose

   Middle entry vector containing QFGal4

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QFGal4
   

   Use

   Gateway middle entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p11d-sctRPA

   Plasmid

   #102614

   Purpose

   Expression of S. cerevisiea RPA in E.coli

   Depositor

   Marc Wold

   Article

   Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

   Inserts

   RPA1 (RFA1 Budding Yeast)
   RPA2 (RFA2 Budding Yeast)
   RPA3 (RFA3 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   RFA2 has intron deleted

   Promoter

   T7

   Available since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-3HA-His3MX6

   Plasmid

   #41600

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-Basic-IRES

   Plasmid

   #64784

   Purpose

   Luciferase reporter vector

   Depositor

   Joshua Mendell

   Article

   Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. UAS:Zebrabow-B

    Plasmid

    #118216

    Purpose

    For broad gal4 inducible expression of Brainbow in zebrafish

    Depositor

    Albert Pan, Alex Schier

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS4333 yeTadA1.0-T7 RNAP

    Plasmid

    #137735

    Purpose

    pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

    Depositor

    Christina Smolke

    Article

    Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

    Insert

    yeTadA1.0-T7 RNAP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    E366G (See depositor comment below)

    Promoter

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAM-kan-R4R3

    Plasmid

    #71275

    Purpose

    Binary destination vector for plant transformation. Kanamycin resistance.

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Type

    Empty backbone

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a MISTIC

    Plasmid

    #85999

    Purpose

    bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    N/A
    

    Use

    Tags

    HIS6 and MISTIC

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LT2_7_OxPro

    Plasmid

    #177709

    Purpose

    Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTRX2_5xUAS-ymYPET-tCYC1
    pTEFmut8-mCherry-tADH1
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLifeAct-miRFP670nano

    Plasmid

    #127434

    Purpose

    F-Actin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    LifeAct-miRFP670nano
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GAL-Aur1-2GFP-Hygromycin

    Plasmid

    #184775

    Purpose

    Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    proGAL-Aur1 (AUR1 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pR26 GFP Dest

    Plasmid

    #74283

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGSHU

    Plasmid

    #72244

    Purpose

    Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    hyg
    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MD-2

    Plasmid

    #20864

    Depositor

    Jie-Oh Lee

    Article

    Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

    Insert

    Myeloid differentiation factor 2 (LY96 Human)
    

    Use

    Tags

    protein A tag

    Expression

    Insect

    Mutation

    contains amino acids 19–160

    Promoter

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)

    Plasmid

    #86418

    Purpose

    ONM/ER split GFP1-10

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-GFP1-10-Scs2TM
    

    Use

    Tags

    GFP1-10

    Expression

    Yeast

    Mutation

    Promoter

    NOP1

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-pEF1a-tTAc

    Plasmid

    #172123

    Purpose

    Constitutive expression of InteinC-tTAC

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-tTAc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pZH82 pET28a-Rai1

    Plasmid

    #231651

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    rai1 (RAI1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZH23 Dual G-less containing ySSA1 polyA

    Plasmid

    #231647

    Purpose

    yeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassette

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    SSA1 polyA termination region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZH20 Dual G-less containing yCYC1 polyA

    Plasmid

    #231646

    Purpose

    yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    CYC1 polyA termination region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid

    #193315

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    

    Use

    Tags

    4FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNMT

    Plasmid

    #42578

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    N-myristoyl tranferase (NMT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations T690C and T942C

    Promoter

    T7

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pLPCX-NICD

    Plasmid

    #44471

    Depositor

    Ernesto Canalis

    Article

    Zanotti et al J Biol Chem. 2011 Feb 11;286(6):4576-88.

    Insert

    Notch intracellular domain (Notch1 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains amino acid 1753 to C-Term

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pRS423-GPD-wrmScarlet1-10-CYC1 terminator

    Plasmid

    #158584

    Purpose

    Expresses wrmScarlet1-10 in S. cerevisiae

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet1-10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pME-Gal80ts

    Plasmid

    #51619

    Purpose

    vertebrate expression of full-length temperature-sensitive Gal80 with stop

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    Gal80ts (GAL80 Budding Yeast)
    

    Use

    Vertebrate expression

    Tags

    Expression

    Mutation

    temperature sensitive (G203V, G323R)*

    Promoter

    Available since

    March 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRISPEY-GAL-Nat

    Plasmid

    #232097

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge