We narrowed to 4,419 results for: 424

1. ATX3-77Q-R388G

   Plasmid

   #184251

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Human, Synthetic)
   

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   C-terminal codon optimization for protein expres…

   Promoter

   T7 promoter

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_NRAS_WT

   Plasmid

   #82150

   Purpose

   Gateway Donor vector containing NRAS , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NRAS (NRAS Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-BLAST flag-CAD S1396A

   Plasmid

   #188126

   Purpose

   Expresses C-terminal flag-tagged human CAD S1396A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. NES-EGFP-PH-BTKx1

   Plasmid

   #183651

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Human, Frog)
   

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 2-170

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-IDH1-P33S

   Plasmid

   #116409

   Purpose

   Lentiviral expression of IDH1 P33S

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   IDH1 (IDH1 Human)
   

   Use

   Lentiviral

   Mutation

   P33S

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTR-341 pUC19-tNGFR-P2A-PDCD1 N

   Plasmid

   #186074

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   PDCD1 (PDCD1 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. R713-M52-303: CMV51p> Halotag-NRasHVR

   Plasmid

   #159686

   Purpose

   Mammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusion

   Depositor

   Dominic Esposito

   Article

   Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

   Insert

   Halotag-NRasHVR (NRAS Human)
   

   Tags

   Halotag

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-myc-AP1S3 F4C

   Plasmid

   #58293

   Purpose

   mammalian expression of AP1S3 F4C mutant

   Depositor

   Francesca Capon

   Article

   Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

   Insert

   AP1S3 (AP1S3 Human)
   

   Tags

   6xHis and myc

   Expression

   Mammalian

   Mutation

   F4C: c.11T>G, p.Phe4Cys

   Promoter

   CMV

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLKO.1-TRC.mKO2_shGLS

   Plasmid

   #110319

   Purpose

   shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   GLS Glutaminase (GLS Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q25

    Plasmid

    #111743

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q25 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-myc-AP1S3 R33W

    Plasmid

    #58294

    Purpose

    mammalian expression of AP1S3 R33W mutant

    Depositor

    Francesca Capon

    Article

    Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

    Insert

    AP1S3 (AP1S3 Human)
    

    Tags

    6xHis and myc

    Expression

    Mammalian

    Mutation

    R33W: c.97C>T,p.Arg33Trp

    Promoter

    CMV

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

12. mRFP-FKBP-INPP5E(D556A)

    Plasmid

    #183678

    Purpose

    Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    INPP5E (INPP5E Human)
    

    Tags

    mRFP-FKBP12(1-108)

    Expression

    Mammalian

    Mutation

    C641A, D556A

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM)

    Plasmid

    #117139

    Purpose

    Expression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFP

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    HA-Dmap1 (mutagenised) (Dmap1 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    modified BFP, modified Dmap1 CDS

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_FAIM_WT_V5

    Plasmid

    #82930

    Purpose

    Gateway Donor vector containing FAIM, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, Matthew Meyerson

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    FAIM (FAIM Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q85

    Plasmid

    #111736

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q85 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO/Strep-HA-ZAKalpha_deltaS

    Plasmid

    #141197

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaS in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of amino acids 670-712

    Promoter

    CMV

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Atg7_L (OZ557)

    Plasmid

    #28082

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting Atg7 (atg7 Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

18. Atg7_R (OZ558)

    Plasmid

    #28083

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting Atg7 (atg7 Zebrafish)
    

    Use

    Zebrafish targeting

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

19. TTN gRNA (BRDN0001145502)

    Plasmid

    #76851

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TTN

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TTN (TTN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. TTN gRNA (BRDN0001148405)

    Plasmid

    #76852

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TTN

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TTN (TTN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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