We narrowed to 3,785 results for: Atr;

1. pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

   Plasmid

   #184636

   Purpose

   Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gat1 (Slc6a1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Gat1 gene with Arginine 69 changed to Lysine, to …

   Promoter

   EF1a

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJET-62-SFFV-T2A-PuroR-polyA-R11b

   Plasmid

   #179893

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJET-64-SFFV-T2A-BlaR-polyA-R11b

   Plasmid

   #179895

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+MLS-HyPer7

   Plasmid

   #136470

   Purpose

   Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Tandem mitochondrial targeting signal of cytochro…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1 Full length Importin beta

   Plasmid

   #106941

   Purpose

   Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

   Depositor

   Patrizia Lavia

   Article

   Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

   Insert

   human Importin beta-1 (KPNB1) (KPNB1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICE-RNaseHI-D10R-E48R-NLS-mCherry

   Plasmid

   #60367

   Purpose

   Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Insert

   RNase HI-D10R-E48R
   

   Use

   Tags

   NLS from SV40 T antigen and mCherry

   Expression

   Mammalian

   Mutation

   D10R+E48R; catalyticaly inactive

   Promoter

   CMV-tet

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. AGTR1-Tango

   Plasmid

   #66222

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   AGTR1 (AGTR1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCWXPGR-pTF-dnTCF4

   Plasmid

   #114277

   Purpose

   TET-inducible expression of a dominant negative form of the TCF4 transcription factor

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   deletion of AA 1 to 31

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pclbw-opa1(isoform 7DeltaS1)-myc

   Plasmid

   #62846

   Purpose

   Mammalian Expression of myc-tagged Opa1 isoform 7 Delta S1

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   mmOPA1isoform7 DeltaS1 - myc (Opa1 Mouse)
   

   Use

   Retroviral

   Tags

   9xMyc

   Expression

   Mammalian

   Mutation

   Promoter

   cmv-bactin

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-Tox2-IRES-eGFP

    Plasmid

    #165428

    Purpose

    Expresses TOX2 in mammalian cells

    Depositor

    Patrick Hogan, Anjana Rao

    Article

    Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

    Insert

    TOX2 (Tox2 Mouse)
    

    Use

    Tags

    3xflag 5' of Tox2

    Expression

    Mammalian

    Mutation

    Promoter

    5' LTR

    Available since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. BasiginCD4d3+4-Comp-His-Stop

    Plasmid

    #36147

    Purpose

    EXPRESs plasmid for human erythrocyte encoding BSG/EMMPRIN/CD147 and CD4d3+4-Comp-His-Stop pentamer

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Basigin (BSG Human)
    

    Use

    Tags

    His tag, pentamerization domain of rat cartilagin…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

    Plasmid

    #201217

    Purpose

    pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    Flag and SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTarget_AT1R-tTA

    Plasmid

    #222851

    Purpose

    Mammalian expression plasmid encoding the human Angiotensin II type I receptor fused to the tTA transcriptional activator

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT1R (AGTR1 Human)
    

    Use

    Tags

    FLAG and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1

    Plasmid

    #10880

    Depositor

    Patrick Aebischer

    Article

    Raoul et al Nat Med. 2005 Apr . 11(4):423-8.

    Insert

    H1-shSOD1 (SOD1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pICE-HA-CtIP-siR-N289A-H290A

    Plasmid

    #82032

    Purpose

    Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICE-HA-CtIP-siR-S664A-S679A-S745A

    Plasmid

    #82031

    Purpose

    Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Non phosphorylatable mutations on 3 of the main A…

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. GUT BuGZ delta ZF1, delta ZF2

    Plasmid

    #84027

    Purpose

    Expression of GFP tagged mutant BuGZ in human cells

    Depositor

    Patrick Paddison

    Article

    Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

    Insert

    BuGZ (ZNF207 Human)
    

    Use

    Lentiviral

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    delta 13-58; Q394R

    Promoter

    UCOE / SFFV

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch

    Plasmid

    #10881

    Depositor

    Patrick Aebischer

    Article

    Raoul et al Nat Med. 2005 Apr . 11(4):423-8.

    Insert

    H1-shSOD1mismatch (SOD1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pJET-49-DExogron-mNeonGreen-R11a

    Plasmid

    #179903

    Purpose

    Donor with homologous arms for Rab11a to knock in DExogron (=minilAA7-DExCon) mNeonGreen module

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    minilAA7-mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

    Plasmid

    #233064

    Purpose

    Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    ALFA and HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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