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pCIS.MTRIA-AGT

Plasmid

#184598

Purpose

For expression of a fluorescent sensor for angiotensin II in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Publication

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-AGT

Expression

Mammalian

Promoter

CAG promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-MCH

Plasmid

#184606

Purpose

For expression of a fluorescent sensor for melanin-concentrating hormone in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Publication

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-MCH

Expression

Mammalian

Promoter

CAG promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NE

Plasmid

#184608

Purpose

For expression of a fluorescent sensor for norepinephrine in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Publication

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NE

Expression

Mammalian

Promoter

CAG promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-OX

Plasmid

#184615

Purpose

For expression of a fluorescent sensor for orexin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Publication

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OX

Expression

Mammalian

Promoter

CAG promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-C3a

Plasmid

#184601

Purpose

For expression of a fluorescent sensor for C3a complement in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Publication

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-C3a

Expression

Mammalian

Promoter

CAG promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-CCK

Plasmid

#184602

Purpose

For expression of a fluorescent sensor for CCK in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Publication

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-CCK

Expression

Mammalian

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NPFF

Plasmid

#184612

Purpose

For expression of a fluorescent sensor for neuropeptide FF in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Publication

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NPFF

Expression

Mammalian

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183605

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Publication

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Promoter

CAG

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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HMGN1_pLENTI-CAG-IRES-GFP

Plasmid

#176995

Purpose

Mammalian lentiviral expression vector encoding HMGN1

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

HMGN1 (HMGN1 Human)

Use

Lentiviral

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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MIS18A_pLENTI-CAG-IRES-GFP

Plasmid

#176996

Purpose

Mammalian lentiviral expression vector encoding MIS18A

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

MIS18A (MIS18A Human)

Use

Lentiviral

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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TRAPPC10_pLENTI-CAG-IRES-GFP

Plasmid

#176998

Purpose

Mammalian lentiviral expression vector encoding TRAPPC10

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

TRAPPC10 (TRAPPC10 Human)

Use

Lentiviral

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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DsRed2_pLENTI-CAG-IRES-GFP

Plasmid

#177010

Purpose

Mammalian lentiviral expression vector encoding DsRed2

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DsRed2

Use

Lentiviral

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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GNAS_pLENTI-CAG-IRES-GFP

Plasmid

#176991

Purpose

Mammalian lentiviral expression vector encoding GNAS

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GNAS (GNAS Human)

Use

Lentiviral

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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Sufu_pLENTI-CAG-IRES-GFP

Plasmid

#176993

Purpose

Mammalian lentiviral expression vector encoding Sufu

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

Sufu (Sufu Mouse)

Use

Lentiviral

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 WT

Plasmid

#182046

Purpose

expression of Sox2

Depositor

Hyonchol Jang

Publication

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Flex-Rev-TdTomato

Plasmid

#122501

Purpose

Cre-dependent TdTomato expression.

Depositor

Kimberly Ritola

Insert

TdTomato

Use

AAV

Promoter

CAG

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PB-ReNL

Plasmid

#123138

Purpose

Piggybac transposon plasmid for live animal optical imaging of Red-enhanced nanolantern

Depositor

Jordan Green

Publication

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Red-enhanced Nanolantern

Expression

Mammalian

Promoter

CAG

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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JEH127

Plasmid

#179299

Purpose

Expresses dCas9 fused to VPR

Depositor

Keith Joung

Publication

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-VPR

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PE SpG

Plasmid

#179081

Purpose

NOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variant

Depositor

Tobias Cantz

Publication

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R

Promoter

CAG

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-IntCCCre-EF1a-Cerulean

Plasmid

#160508

Purpose

Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Publication

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

IntCCCre

Use

Cre/Lox and Lentiviral

Tags

mCerulean

Expression

Mammalian

Promoter

CAG

Available since

Jan. 22, 2022

Availability

Academic Institutions and Nonprofits only

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