We narrowed to 5,622 results for: crispr cas9 grna plasmid

1. cTRE-LSL-GFP-IRES-Cas9-sgCR8

   Plasmid

   #135668

   Purpose

   Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   sgCR8
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

   Plasmid

   #135669

   Purpose

   Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   sgPten
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS1099: Dual-sgRNA.Design 4

   Plasmid

   #159537

   Purpose

   Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

   Insert

   Nme2Cas9 with two guide RNA cassettes
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-(BbsI)_CBh-Cas9-T2A-BFP

   Plasmid

   #64323

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

   Plasmid

   #199580

   Purpose

   Expresses GCaMP8f in a Cre-dependent manner

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   His-DIO GCaMP8f
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hsyn

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

   Plasmid

   #160571

   Purpose

   Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNAscaffold 2.1 scRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKDsgRNA-p15

   Plasmid

   #62656

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

    Plasmid

    #215281

    Purpose

    Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Grin1 gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    U6

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX552-hSyn-DIO-GCaMP8f_Grin1TTT gRNA

    Plasmid

    #215282

    Purpose

    Expresses Grin1 Control gRNA in a Cre dependent GCaMP8f vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Grin1 control gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICH86966::AtU6p::sgRNA:NbVPE1a/b

    Plasmid

    #223218

    Purpose

    Expresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoter

    Depositor

    Tsepo Tsekoa

    Article

    Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

    Insert

    NbVPE1a/b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6 promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Puro (PX459) V2.0

    Plasmid

    #62988

    Purpose

    Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9-2A-Puro V2.0
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC154-dual-dCas9VP160-sgExpression

    Plasmid

    #48240

    Purpose

    Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A H840A

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC152-dual-dCas9VP64-sgExpression

    Plasmid

    #48238

    Purpose

    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA Tag, HA-Tag, and VP64

    Expression

    Mammalian

    Mutation

    D10A;H840A

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC153-dual-dCas9VP96-sgExpression

    Plasmid

    #48239

    Purpose

    Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP96

    Expression

    Mammalian

    Mutation

    D10A H840A

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCBFKOgRNA5

    Plasmid

    #84366

    Purpose

    Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

    Depositor

    Danny Reinberg

    Article

    Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

    Insert

    Cbfa2t2-gRNA5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

19. mCBFKOgRNA2

    Plasmid

    #84365

    Purpose

    Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

    Depositor

    Danny Reinberg

    Article

    Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

    Insert

    Cbfa2t2-gRNA2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

20. hIRF-1 gRNA #409 (Sa)

    Plasmid

    #98134

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    gRNA_hIRF1 promoter #409
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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