We narrowed to 5,300 results for: pcDNA3.1+
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pcDNA_DiCre_60.F2
Plasmid#60208PurposeExpresses a fusion protein of FRB-linker peptide 2- Cre(60-343)DepositorInsertCre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
UseTagsFRAP and Nuclear localisation signalExpressionMammalianMutationamino acids 2021-2112 of FRAP with a T2098L mutat…PromoterCMVAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCmR2
Plasmid#59015PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-PP7-ccdB
Plasmid#99912PurposeBasic cloning vector for the expression of PP7 inserted sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
mGFAP-Halo
Plasmid#169474Purposethe host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sitesDepositorInsertGFAP (Gfap Mouse)
UseTagsHaloTagExpressionMammalianMutationChanged R 236 to HPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8f F366H
Plasmid#204141PurposeFluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutationDepositorInsertjGCaMP8f F366H
UseTagsN-terminal His tagExpressionMammalianMutationPhenylalanine 366 changed to histidinePromoterCMVAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-MS2-ccdB
Plasmid#99911PurposeBasic cloning vector for the expression of MS2 inserted sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Bongwoori
Plasmid#63720PurposeConsists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227DDepositorInsertBongwoori
UseTagsSuper ecliptic pHlorin A227DExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX
Plasmid#188993PurposeA plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationD201G, A258T, K327R, G328S, G381V, G429D, T450APromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-NLS-mut
Plasmid#42000DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationR930A, R931A, R932A, R934APromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSSA-PMG (pXK-1649)
Plasmid#208857PurposeSSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-SCW-HHL
Plasmid#190634PurposeMammalian expression of genetically encoded calcium indicator SCW-HHLDepositorInsertSCW-HHL
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PA-RL-mCherry
Plasmid#78861PurposeDULIP negative control (bait) to exclude unspecific background bindingDepositorInsertmCherry
UseExpression vectorTagsProtein A, Renilla luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-CAML
Plasmid#22284DepositorInsertcalcium-modulating ligand (CAMLG Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dMASS_1
Plasmid#209764PurposeExpression of dMASS_1 in mammalian cells under CMV promoterDepositorInsertdMASS_1
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670
Plasmid#169591PurposeTier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).DepositorInserttetracycline-controlled SEAP and iRFP expression
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVmin-2Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
b2-TS3
Plasmid#103877Purposefull-length human b2 with tension sensor at b2_733DepositorInsertfull-length human b2 with tension sensor at b2_733 (ITGB2 Human)
UseTagstension sensor moduleExpressionMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCY2
Plasmid#59016PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
aL-mEos3.2
Plasmid#113154Purposeconstrained LFA-1-mEos3.2 fusionDepositorInsertfull-length human aL with mEOS3.2 in betapropeller (ITGAL Human)
UseTagsmEOS3.2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 4
Plasmid#36114DepositorInsertG protein beta subunit B4 (Gnb4 Mouse)
UseTagsmcherryExpressionMammalianMutationPromotercmvAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only
