We narrowed to 9,400 results for: Coli

1. pEcoFus

   Plasmid

   #187833

   Purpose

   1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpression

   Depositor

   Jörg Vogel

   Article

   Ponath et al Nat Microbiol. 2021 Aug;6(8):1007-1020.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSG86-J23151-LasR

   Plasmid

   #102456

   Purpose

   Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   LasR (lasR )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23151

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBlueKan+cysMPro

   Plasmid

   #187879

   Purpose

   Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

   Depositor

   Gaylen Uhlich

   Article

   Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CysM

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAL-c5X-ELP[AV-60]

   Plasmid

   #67012

   Purpose

   Expresses malE-ELP[AV-60] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-ELP[AV-60]
   

   Use

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pG1AK-sfGFP

   Plasmid

   #71739

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRplsWT

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b_pScaC-SNAP_6xHis

   Plasmid

   #228835

   Purpose

   E. coli expression of the SNAP-tagged ScaC passenger domain

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaC
   

   Use

   Tags

   SNAPtag-TEV-6xHis

   Expression

   Bacterial

   Mutation

   aa 33-223

   Promoter

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet1_CylLS_CylM

   Plasmid

   #208760

   Purpose

   Expresses modified cytolysin S (mCylLS) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CylLS
   CylLM
   

   Use

   Tags

   Hisx6 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGTetRnls

   Plasmid

   #26599

   Depositor

   Peter Andrews

   Article

   Zafarana et al Stem Cells. 2009 Apr . 27(4):776-82.

   Insert

   Tetracycline repressor (TetR E.Coli)
   

   Use

   Tags

   nls

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. MK1283

   Plasmid

   #71428

   Purpose

   This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

   Depositor

   Howard Salis

   Article

   Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

   Insert

   Mixed Feedback Loop version of the UBER system
   

   Use

   Tags

   Expression

   Mutation

   T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

   Promoter

   Available Since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSETA_crGE

    Plasmid

    #172927

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

    Depositor

    Arnold Boersma

    Article

    Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

    Insert

    CrowdingSensorGE
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PtxS1-c003

    Plasmid

    #173076

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PtxS1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D35-I221

    Promoter

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMAL-c5X-ELP[V-60]

    Plasmid

    #67013

    Purpose

    Expresses malE-ELP[V-60] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-ELP[V-60]
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSG22-pBAD-TetR

    Plasmid

    #102451

    Purpose

    Expresses TetR protein in E. coli. The expression is controlled by a pBAD promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    TetR (tetR )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-6xHis-GFP(0)

    Plasmid

    #199166

    Purpose

    used to express a neutral GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(0)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB1C3-J23110-B0034-mRFP1_Violet

    Plasmid

    #160447

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1E_Violet
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKFSS1_2

    Plasmid

    #118227

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector; streptomycin resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBSV2G_2

    Plasmid

    #118225

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector, gentamicin resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSG28-J23151-AraC

    Plasmid

    #102453

    Purpose

    Expresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    AraC (araC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23151

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Pet28a-His-tag-BS2

    Plasmid

    #220347

    Purpose

    E. coli expression vector to purify BS2

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pSB1C3-J23110-B0034-mRFP1E

    Plasmid

    #160442

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Salmonella MTAN

    Plasmid

    #64041

    Purpose

    Expression of S. enterica MTAN in E. coli

    Depositor

    Vern Schramm

    Article

    Hemeon et al Anal Chem. 2011 Jun 15;83(12):4996-5004.

    Insert

    Methylthioadenosine nucleosidase
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pOGG005

    Plasmid

    #113980

    Purpose

    Level 1 high copy number vector for E. coli expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSETb-Ferritin-Dronpa

    Plasmid

    #101194

    Purpose

    Expresses a Ferritin-Dronpa fusion construct in E. coli

    Depositor

    Peter Dedecker

    Article

    Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

    Insert

    Ferritin-Dronpa
    

    Use

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MK1274

    Plasmid

    #71431

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Use

    Tags

    Expression

    Mutation

    T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…

    Promoter

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUMV19

    Plasmid

    #67161

    Purpose

    inducible operation of the mevalonate pathway in E. coli

    Depositor

    Tomohisa Kuzuyama

    Article

    Takagi et al J Bacteriol. 2000 Aug;182(15):4153-7.

    Insert

    the mevalonate pathway genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available Since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-4T1 mouse PARG (439-959)

    Plasmid

    #132615

    Purpose

    expresses mouse PARG(439-959) in E. coli.

    Depositor

    Wenqing Xu

    Article

    Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

    Insert

    PARG (439-959)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL4.26-SS-352

    Plasmid

    #68791

    Purpose

    ZF9 Op x6 -- GFP-IRES-NTR

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    ZF9 Op 6x
    EGFP
    Nitroreductase
    

    Use

    Tags

    IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET51b_SNAP-ecDHFR

    Plasmid

    #187108

    Purpose

    Expression of SNAP-ecDHFR fusion in bacteria

    Depositor

    Kai Johnsson

    Article

    Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

    Insert

    SNAP-ecDHFR
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNIC-CFP

    Plasmid

    #173074

    Purpose

    Expression vector for E. coli producing target protein with N-terminal CFP fusion

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Type

    Empty backbone

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-6xHis-GFP(+6)

    Plasmid

    #199160

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+6)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge