We narrowed to 8,624 results for: FIE
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Plasmid#183694PurposeCRISPRa gRNA expression vector for Chinese Hamster Bip (Hspa5)DepositorInsertgRNA targeting chinese hamster Bip (Hspa5)
UseCRISPRAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTij_sg_mmu_Med6_C_terminal
Plasmid#197869PurposeCRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCsDepositorAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2X(CMV-eBFP2-3XNLS-pA)
Plasmid#195867Purposeto express nuclear localized eBFP2DepositorInserteBFP2
UseAAVPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No8
Plasmid#194902PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-8 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.8
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No6
Plasmid#194900PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-6 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.6
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No7
Plasmid#194901PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-7 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.7
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDe_Cas9_KANA_R160a
Plasmid#173756PurposeBinary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160aDepositorInsertCas9_sgRNA1/R160a_sgRNA2/R160a
UseCRISPRPromoterAtU6 &StU6Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDe_Cas9_KANA_R160b
Plasmid#173757PurposeBinary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160bDepositorInsertCas9_sgRNA1/R160b_sgRNA2/160b
UseCRISPRPromoterAtU6 &StU6Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDe_Cas9_KANA_R390a
Plasmid#173758PurposeBinary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390aDepositorInsertCas9_sgRNA1/390a_sgRNA2/390a
UseCRISPRPromoterAtU6 &StU6Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-UTX(420-633)-H6
Plasmid#183773PurposeExpress pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.DepositorInsertUTX
Tags6 His tag at the C-terminusExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-SU(Z)12(624-900)-H6
Plasmid#183772PurposeExpress of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.DepositorInsertSU(Z)12 residues 624-900 (Su(z)12 Fly)
TagsHis tag at the C-terminusExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
gMUM2-Citrine
Plasmid#181965PurposeMUM2 protein fused to Citrine, under control of native promoterDepositorAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0061
Plasmid#177026PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsertiridoid oxidase; CYP76A26
UseSynthetic BiologyMutationamplified from cDNA, contains T359S relative to K…Available SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
YF
Plasmid#171459PurposeExpresses YF, a FiCE of iCMM, in mammalian cellsDepositorInsertEYFP-FKBP12
ExpressionMammalianPromoterCMVAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH–PB
Plasmid#169685PurposeAn anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody
Tagsmodified STIM1-PB tagExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159961PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159965PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterCMV-IEAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159964PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterCMV-IEAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159958PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159957PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159960PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159959PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE
Plasmid#159963PurposeoptoNES construct with modified mTurquoise2-LOV2 RETDepositorInsertNLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21
UseAAVExpressionMammalianMutationsyntheticPromoterCMV-IEAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDA3::HA exon2 (WS13)
Plasmid#126874Purposep3xHA inserted within second exon of IDA3. Includes ampicilin and hygromycin resistance.DepositorInsertIDA3
UseOtherAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDA3::NeonGreen N-term (WS14)
Plasmid#126875PurposeNeonGreen-tag attached to the N-terminus of IDA3. Includes ampicilin and hygromycin resistance.DepositorInsertIDA3
UseOtherTagsNeonGreenAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDA3::NeonGreen exon2 (WS15)
Plasmid#126876PurposeNeonGreen-tag embedded in the second exon of IDA3. Includes ampicilin and hygromycin resistance.DepositorInsertIDA3
UseOtherAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis-Laforin
Plasmid#157989PurposeExpresses Laforin in E.coli cellDepositorAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXW109ABS62(RS)
Plasmid#123153PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS62, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXW109ABS67(RS)
Plasmid#123154PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109. ParsR-ABS67, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD2029
Plasmid#101372PurposeName: pBluescript-LANApiΔTATA. LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000).DepositorInsertLANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000).
UseUnspecifiedMutationLANApi TATA box mutation in pBluescript-LANApi,K1…Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2030
Plasmid#101373PurposeName: pBluescript-RBPjkΔB. RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).DepositorInsertRBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).
UseUnspecifiedMutationRBPjk binding site B mutation in pBluescript-LANA…Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2031
Plasmid#101374PurposeName: pBluescript-RBPjkΔA. RBPjk binding site A mutation in pBluescript-LANApi,K14 (pDD2000).DepositorInsertRBPjk binding site A mutation in pBluescript-LANApi,K14 (pDD2000).
UseUnspecifiedMutationRBPjk binding site A mutation in pBluescript-LANA…Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMYA5T2/pENTR3C
Plasmid#97409PurposeCMYA5 (NM_153610, nt 12205 to 12279) T allele (rs10043986) in pENTR3C, Gateway Entry vectorDepositorAvailable SinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMYA5T/pBSSK+
Plasmid#97206PurposeCMYA5 (NM_153610, nt 12205 to 12279) T allele (rs10043986) in pBSSK+DepositorAvailable SinceAug. 3, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTIM
Plasmid#81198PurposepTIM in vitro transcription backbone plasmid with two multiple cloning sites and two Rho-independent transcriptional terminators.DepositorTypeEmpty backboneUseUnspecifiedAvailable SinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha1_SF (GB0106)
Plasmid#68232PurposeTwister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVK
Plasmid#66072PurposeMoClo Destination Vector: Empty vector for creating new DVK. Cut with SpeI and insert amplified LacZa (based on pSB1K3)DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVA
Plasmid#66056PurposeMoClo Destination Vector: Empty vector for creating new DVA. Cut with SpeI and insert amplified LacZa (based on pSB1A2)DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB1omega1_SF (GB0158)
Plasmid#68242PurposeTwister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedMutationBsaI and BsmBI sites removedAvailable SinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEW-Venus-Sec16A-deltaN
Plasmid#66605PurposeLentiviral vector for express truncated form of Sec16A as a fusion with Venus FPDepositorInsertSec16A (SEC16A Human)
TagsVenus (EYFP derivative)ExpressionMammalianMutationencodes amino acids 1107-1217 of human Sec16AAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only