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We narrowed to 8,240 results for: FIE

Showing: 7521 - 7560 of 8240 results

  2. pFRT-TODestFLAGHA_RBPMS_KDR_111del

    Plasmid
    #65659
    Purpose
    FLAG-HA expression vector with RBPMS carrying deletion at amino acid 111 (1-111) and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    introduced stop codon such as to produce deletion…
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65659-plasmid-map-sequence-id-111636

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  3. pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A

    Plasmid
    #65660
    Purpose
    FLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    changed glutamic acid 97 to alanine, lysine 100 t…
    Promoter
    CMV, 2x Tet
    Available since
    June 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    65660-plasmid-map-sequence-id-111635

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  5. pF(UG) hSyn NS34a-msGFP-A'Q-mito

    Plasmid
    #158766
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    158766-plasmid-map-sequence-id-313130

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  6. pF(UG) hSyn NS34a-msGFP-'Q-mito

    Plasmid
    #158767
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    158767-plasmid-map-sequence-id-313145

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  14. Lamp1-RFP

    Plasmid
    #1817
    Purpose
    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Article
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Promoter
    Available since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only
    1817-plasmid-map-sequence-id-350820

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  18. CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

    Plasmid
    #140892
    Purpose
    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.
    Depositor
    Yasushi Okamura
    Article
    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1
    Insert
    CiDr-VSP L223F-mCherry (tpte, vsp Zebrafish, Ciona intestinalis)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed 223L to F in Dr-VSP (zebrafish TPTE)
    Promoter
    Available since
    April 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    140892-plasmid-map-sequence-id-292709

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  21. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

    Plasmid
    #89888
    Purpose
    Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d
    Insert
    mCherry-KRasG12V
    Use
    Unspecified
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89888-plasmid-map-sequence-id-181093

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  27. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid
    #55200
    Purpose
    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55200-plasmid-map-sequence-id-85587

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  28. pKrox24(MapErk)Luc

    Plasmid
    #200112
    Purpose
    Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    MapErk sequence in minimal promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    highly modified sequence
    Promoter
    5 copies of designed MapErk sequence in minimal p…
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    200112-plasmid-map-sequence-id-399196

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  31. pH-ABE8e

    Plasmid
    #200774
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9(D10A)-TadA8e
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200774-plasmid-map-sequence-id-399597

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  32. GST-mDia1(FH1FH2DAD)-His

    Plasmid
    #85822
    Purpose
    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
    Depositor
    Guillaume Romet-Lemonne, Antoine Jégou
    Article
    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc
    Insert
    Protein diaphanous homolog 1 (Diaph1 Mouse)
    Use
    Tags
    8*His and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85822-plasmid-map-sequence-id-183287

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  33. pEM-Cas9HF1

    Plasmid
    #89961
    Purpose
    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
    Depositor
    Michael Lynch
    Article
    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021
    Insert
    Cas9HF1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N497A/R661A/Q695A/Q926A
    Promoter
    pTet
    Available since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    89961-plasmid-map-sequence-id-181339

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  38. PB-XL-AncBE4max

    Plasmid
    #136254
    Purpose
    Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max
    Depositor
    Zhaohui Ye
    Article
    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:
    Insert
    AncBE4max_GFP
    Use
    CRISPR; Piggybac dna transposon
    Tags
    Expression
    Mammalian
    Mutation
    The AncBE4max_GFP insert was PCR amplified from a…
    Promoter
    TRE3G
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136254-plasmid-map-sequence-id-270570

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Showing: 7521 - 7560 of 8240 results