We narrowed to 9,372 results for: bli

1. AAV-sCAG-eGFP-ACAP1

   Plasmid

   #203800

   Purpose

   AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Insert

   ACAP1 (ACAP1 Human)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDB179-BhrPETase

   Plasmid

   #218692

   Purpose

   Encodes codon optimized BhrPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   BhrPETase
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti_AID-Dam-lamin B1

   Plasmid

   #119764

   Purpose

   Lentiviral plasmid for Mammalian Expression

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Borsos et al Nature. 2019 May;569(7758):729-733. doi:

   Insert

   AID-Dam-lamin B1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

   Plasmid

   #66707

   Purpose

   expresses a single gRNA and VP64-dCas9-VP64

   Depositor

   Charles Gersbach

   Inserts

   hU6-gRNA expression cassette
   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Mutation

   D10A and H840A

   Promoter

   hU6 and hUbC

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 human cofilin WT

   Plasmid

   #50853

   Purpose

   expresses human cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 (CFL1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

   Plasmid

   #199781

   Purpose

   Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).

   Depositor

   Sascha Martens

   Insert

   NEMO (IKBKG Human)
   

   Tags

   GST-Thrombin-TEV-EGFP

   Expression

   Bacterial

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 7MEQ

   Plasmid

   #176412

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
   

   Use

   Baculovirus expresssion

   Tags

   8 x His Tag and Honeybee melittin signal

   Mutation

   etmprss2_S251D/R252D/Q253D/S254D/R255K

   Promoter

   Polyhedrin

   Available Since

   Oct. 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. TAF1

   Plasmid

   #39117

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TAF1 (TAF1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. Lenti-DAG1

   Plasmid

   #205149

   Purpose

   Overexpress DAG1

   Depositor

   Monkol Lek

   Insert

   Dystroglycan 1 (DAG1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Carries common missense variant c.41C>G (p.Ser…

   Promoter

   EF-1a

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid

    #50455

    Purpose

    Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFP.E227

    Plasmid

    #138249

    Purpose

    Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    mCherry
    Terminator
    mTagBFP
    Terminator
    gfp
    Terminator
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P17_up1691, P20_992, and iP16_3622

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

    Plasmid

    #216664

    Purpose

    Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

    Depositor

    Leonardo Morsut

    Inserts

    ETV2 (Etv2 Mouse)
    BFP
    HygromycinR
    

    Use

    Lentiviral

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDB179-CaPETaseM9

    Plasmid

    #218707

    Purpose

    Encodes codon optimized CaPETaseM9 enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    CaPETaseM9
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)

    Plasmid

    #125146

    Purpose

    An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

    Depositor

    Christopher Richie

    Insert

    HA-tagged hM4D(Gi) (CHRM4 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Promoter

    SYN1

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AICSDP-166:CDH1-mEGFP

    Plasmid

    #193921

    Purpose

    Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1

    Depositor

    Allen Institute for Cell Science

    Insert

    cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
    

    Use

    AAV and CRISPR; Donor template

    Tags

    linker-mEGFP

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET29b Tau Cys

    Plasmid

    #108867

    Purpose

    Expresses human Tau with a mutated cysteine

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Expression

    Bacterial

    Mutation

    I260C C291A C322A to allow maleimide dye labelling

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRN3P_membrane_EGFP

    Plasmid

    #139402

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    membrane-EGFP
    

    Use

    In vitro transcription

    Tags

    EGFP

    Promoter

    T3

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AICSDP-60: SOX2-mEGFP

    Plasmid

    #124606

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SOX2

    Depositor

    Allen Institute for Cell Science

    Insert

    SOX2 Homology Arms with linker-mEGFP (SOX2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    May 13, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pTipi2.1_Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC

    Plasmid

    #221361

    Purpose

    Mammalian expression of Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC; to be used with Anti-ProC [IPI-HPC-4] light chain (Plasmid 221362) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pBABE.puro/TO/Flag/tGFP-HuR.wt

    Plasmid

    #110349

    Purpose

    Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tags

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Promoter

    gag

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. DT0

    Plasmid

    #80418

    Purpose

    Expresses 0 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Membrane mNeonGreen

    Plasmid

    #198057

    Purpose

    Plasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequence

    Depositor

    Amro Hamdoun

    Insert

    LCK membrane mNeonGreen (LCK A.victoria, Human)
    

    Use

    Synthetic Biology; Sea urchin, mammal, zebrafish

    Tags

    mNeonGreen

    Mutation

    Tandem repeat of N-terminal LCK membrane targetin…

    Promoter

    sp6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti_AID-Dam-only

    Plasmid

    #119765

    Purpose

    Lentiviral plasmid for Mammalian Expression

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    AID-Dam only
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOD_009

    Plasmid

    #159520

    Purpose

    Rifampicin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa

    Plasmid

    #221352

    Purpose

    Mammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa; to be used with Anti-EE [IPI-3D5EE_48.K] heavy chain (Plasmid 221351) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC

    Plasmid

    #221351

    Purpose

    Mammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC; to be used with Anti-EE [IPI-3D5EE_48.K] light chain (Plasmid 221352) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. AICSDP-80:UBTF-HaloTag

    Plasmid

    #133963

    Purpose

    Homology arms and HaloTag-linker sequence for N-terminus tagging of human UBTF

    Depositor

    Allen Institute for Cell Science

    Insert

    UBTF Homology Arms with HaloTag-linker (UBTF Human)
    

    Use

    CRISPR

    Tags

    HaloTag-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRN3P_EGFP_m6A_Tracer

    Plasmid

    #139403

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    m6A-Tracer
    

    Use

    In vitro transcription

    Tags

    EGFP

    Promoter

    T3

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Membrane EGFP

    Plasmid

    #198056

    Purpose

    Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence

    Depositor

    Amro Hamdoun

    Insert

    LCK membrane EGFP (LCK A. victoria, Synthetic, Human)
    

    Use

    Sea urchin, mammal, zebrafish

    Tags

    EGFP

    Mutation

    Tandem repeat of N-terminal LCK membrane targetin…

    Promoter

    sp6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPyCAG-mKate2-3xNLS-IRES-Pac

    Plasmid

    #183618

    Purpose

    Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

    Insert

    mKate2-3xNLS
    

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJ151-HDR

    Plasmid

    #113631

    Purpose

    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

    Depositor

    Alex Kentsis

    Inserts

    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    

    Use

    Cre/Lox; Homology recombination selection vector

    Expression

    Bacterial and Mammalian

    Promoter

    EF1a promoter, EF1a promoter (T2A cleaving sequen…

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti Puro HA-Ubiquitin-K48

    Plasmid

    #236290

    Purpose

    Lentiviral vector expressing HA-tagged ubiquitin K48 mutant

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    Ubiquitin K48 (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    Single ubiquitin coding sequence containing K48 m…

    Promoter

    CMV

    Available Since

    April 10, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pLenti Puro HA-Ubiquitin-K63

    Plasmid

    #236291

    Purpose

    Lentiviral vector expressing HA-tagged ubiquitin K63 mutant

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    Ubiquitin K63 (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    Single ubiquitin coding sequence containing K63 m…

    Promoter

    CMV

    Available Since

    April 10, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. AICSDP-57: SMC1A-mEGFP

    Plasmid

    #114406

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A

    Depositor

    Allen Institute for Cell Science

    Insert

    SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pAAV/hSyn-SCLM

    Plasmid

    #216760

    Purpose

    AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon

    Depositor

    George Augustine

    Insert

    SuperClomeleon
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    hSyn (human synapsin I)

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-osTIR1-9xMyc-Zeo

    Plasmid

    #225696

    Purpose

    Lentiviral expression of 9xMycTag fused osTIR1and Zeocin resistance. For use with AID degron.

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    osTIR1
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    9X Myc

    Expression

    Mammalian

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pQTEV-STMN1

    Plasmid

    #31326

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    stathmin 1/oncoprotein 18 (STMN1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKOR1-mcs

    Plasmid

    #181755

    Purpose

    Allele replacement for Staphylococcus aureus

    Depositor

    Suzan Rooijakkers

    Article

    Stapels et al Proc Natl Acad Sci U S A. 2014 Sep 9;111

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEMTEZ-Kir2.1

    Plasmid

    #32641

    Depositor

    Richard Axel, C. Ron Yu, Joseph Gogos

    Article

    Yu et al Neuron. 2004 May 27;42(4):553-66.

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Expression

    Bacterial

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ITCH

    Plasmid

    #36197

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ITCH (ITCH Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains HECT domain

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits