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Showing: 9401 - 9450 of 17259 results

  30. KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L

    Plasmid
    #121840
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121840-plasmid-map-sequence-id-237394

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  31. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid
    #121803
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + EF1a::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121803-plasmid-map-sequence-id-237312

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  32. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

    Plasmid
    #121807
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + RIP::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121807-plasmid-map-sequence-id-237316

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  33. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

    Plasmid
    #121811
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121811-plasmid-map-sequence-id-237356

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  34. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid
    #121815
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121815-plasmid-map-sequence-id-237362

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  35. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid
    #121820
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121820-plasmid-map-sequence-id-237373

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  37. IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

    Plasmid
    #121748
    Purpose
    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    OLLAS epitope tag-IRES2-eGFP + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121748-plasmid-map-sequence-id-237186

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  44. pCEP4-CH111-QES.c13.L544Y-754*

    Plasmid
    #123264
    Purpose
    Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (AD8) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123264-plasmid-map-sequence-id-235227

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  45. pCEP4-001428-QES.c10.L544Y-754*

    Plasmid
    #123253
    Purpose
    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (001428) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123253-plasmid-map-sequence-id-235211

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  46. pCEP4-AD8-QES.i08.c04-754*

    Plasmid
    #123258
    Purpose
    Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (AD8) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123258-plasmid-map-sequence-id-235223

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  47. pCEP4-001428(753)-VC

    Plasmid
    #123256
    Purpose
    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (001428) Env
    Tags
    CD5 leader peptide and VC (Venus residues D155–K2…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; C-terminal Env re…
    Promoter
    CMV
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123256-plasmid-map-sequence-id-235217

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  48. pCEP4-ZM197M-QES.c10-754*

    Plasmid
    #123222
    Purpose
    Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (ZM197M) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    123222-plasmid-map-sequence-id-235165

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  49. pCEP4-Q842-QES.i04.c05

    Plasmid
    #123233
    Purpose
    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (Q842.d12) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations P124D, …
    Promoter
    CMV
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    123233-plasmid-map-sequence-id-235181

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  50. pCEP4-BaL-QES.i01.c08-754*

    Plasmid
    #123213
    Purpose
    Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (BaL) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    123213-plasmid-map-sequence-id-235153

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Showing: 9401 - 9450 of 17259 results