We narrowed to 5,115 results for: LucY

1. pPLN-hFluc-DM-eGFP-KDEL

   Plasmid

   #177728

   Purpose

   DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFlucDM
   

   Tags

   KDEL, Prolactin Signal Sequence, and eGFP

   Expression

   Bacterial and Mammalian

   Mutation

   R188Q, R261Q (Destabilized Mutant)

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFBD-Akt1(T308A/S473A)

   Plasmid

   #86578

   Purpose

   Expresses human full-length Akt1(T308A/S473A) phosphomutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   T308A/S473A phosphomutant

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM1157

   Plasmid

   #112534

   Purpose

   pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   luxCDABE
   

   Expression

   Bacterial

   Promoter

   PL(HrtO) and ProD

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-FRB:Akt1(D274A)

   Plasmid

   #86634

   Purpose

   Expresses FRB-tagged human Akt1(D274A) kinase-inactive mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   FRB-Akt1(D274A) (AKT1 Human)
   

   Expression

   Mammalian

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIS1-wt RhoA 3'UTR

   Plasmid

   #26089

   Depositor

   Bob Weinberg

   Article

   Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

   Insert

   wt RhoA 3'UTR (RHOA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pmCherry-PH1(K14A)

   Plasmid

   #86600

   Purpose

   Expresses mCherry-tagged PH(K14A) domain of human Akt1

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   K14A mutant of Akt1 PH domain (AKT1 Human)
   

   Expression

   Mammalian

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. -111 hTF m3

   Plasmid

   #15450

   Depositor

   Nigel Mackman

   Article

   Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

   Insert

   Tissue factor promoter (F3 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   All 3 EGR-1 sites mutated

   Available Since

   July 27, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pOPTXcGMPRELUC

   Plasmid

   #68503

   Purpose

   cyclic nucleotide reporter for bacteria or plant cells

   Depositor

   Helen Irving

   Article

   Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

   Insert

   OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
   

   Tags

   luciferase

   Expression

   Bacterial and Plant

   Mutation

   3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

   Promoter

   OPTX promoter with cGMPRE

   Available Since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

9. -111 hTF m4

   Plasmid

   #15451

   Depositor

   Nigel Mackman

   Article

   Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

   Insert

   Tissue factor promoter (F3 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   All 3 Sp1 and EGR-1 sites mutated

   Available Since

   July 27, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pFBD-Akt1(K14A)

    Plasmid

    #86562

    Purpose

    Expresses human full-length Akt1(K14A) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    K14A mutation in the PH domain

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMEH60 Lamp2b MS2 HA

    Plasmid

    #168209

    Purpose

    Mammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    HA and MS2 dimer

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL2 p15 4xSBR2 MutSBE

    Plasmid

    #15707

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    p15 4xSBR2 (CDKN2B Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant SBE (Smad binding element)

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pGL2 p15 4xSBR1 MutSBE

    Plasmid

    #15718

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    p15 4xSBR1 (CDKN2B Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant SBE (Smad binding element)

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

14. psiCheck-ApoE3'UTR

    Plasmid

    #41846

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

    Insert

    ApoE 3'UTR (APOE Human)
    

    Use

    Luciferase

    Available Since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAG179 BC072452 3'UTR mut

    Plasmid

    #12037

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N12 3'UTR mut (FAM107B Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pOPTXGARE

    Plasmid

    #68542

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with GARE (AT1G33440 Mustard Weed)
    

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    5x GARE inserted 190 bp 5' pf ATG (GARE = ta…

    Promoter

    OPTX promoter with GARE

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNB785

    Plasmid

    #60164

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNB787

    Plasmid

    #60152

    Purpose

    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNB789

    Plasmid

    #60166

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    RNR1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG73 PFTK1 3'UTR mut

    Plasmid

    #12061

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    PFTK1 3'UTR mut (CDK14 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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