We narrowed to 584 results for: pcmv gfp

1. apt-ABE8e

   Plasmid

   #219937

   Purpose

   Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Com_93-aa_TadA8e
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Equalizer-L plasmid

   Plasmid

   #169732

   Purpose

   Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Equalizer-H plasmid

   Plasmid

   #169367

   Purpose

   Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Equalizer-M plasmid

   Plasmid

   #169731

   Purpose

   Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. bpNLS-DddIA

   Plasmid

   #187847

   Purpose

   expresses the bpNLS-linked DddIA in mammalian cells

   Depositor

   Chengqi Yi

   Article

   Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

   Insert

   bpNLS-DddIA-bpNLS-p2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. apt-CBE-3'end

   Plasmid

   #219939

   Purpose

   Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   MCP_32-aa_evo-rA1_2xUGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. apt-CBE-SL3

   Plasmid

   #219940

   Purpose

   Mammalian expression of apt-CBE-SL3 protein (MCP_16aa-linker_evoAPOBEC1) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   MCP_16-aa_evo-rA1_2xUGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. miniCGBE1 (pBM1219)

   Plasmid

   #140253

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Equalizer-L episome

   Plasmid

   #169738

   Purpose

   Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ECibN

    Plasmid

    #209670

    Purpose

    Expresses E-cadherin fused with eGFP, CIB1, and Split-TurboID-N in mammalian cells

    Depositor

    Sanjeevi Sivasankar

    Article

    Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

    Insert

    E-Cadherin (CDH1 Canis lupus familiaris)
    

    Use

    Tags

    CIB1, Split-TurboID-N, and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMV+ plasmid with onboard mCherry cassette

    Plasmid

    #169741

    Purpose

    CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. miniCGBE1-NG (pBM1182)

    Plasmid

    #140257

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CGBE1 (pRZ3885)

    Plasmid

    #140252

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. miniCGBE1-VRQR (pBM1173)

    Plasmid

    #140255

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_VRQR-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SECURE BE4max(R33A) (pBM608)

    Plasmid

    #140251

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SPACE-ΔUGI (pRZ1836)

    Plasmid

    #140243

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CGBE1-VRQR (pBM1192)

    Plasmid

    #140254

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_VRQR-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. miniCGBE1-SpRY (pBM1571)

    Plasmid

    #170105

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

    Promoter

    CMV

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. m6A-Tracer-NES

    Plasmid

    #159607

    Purpose

    Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.

    Depositor

    Aaron Streets

    Article

    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

    Insert

    m6A-Tracer-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEYFP-N3-EPAC1

    Plasmid

    #113110

    Purpose

    Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

    Depositor

    Xiaodong Cheng

    Article

    Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

    Insert

    RapGEF3 (RAPGEF3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    PCMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SPACE-NG (pRZ5150)

    Plasmid

    #140244

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

    Promoter

    CMV

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SPACE (pRZ1813)

    Plasmid

    #140242

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SPACE-VRQR (pRZ5137)

    Plasmid

    #140245

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_VRQR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, VRQR mutations in SpCas9, D10A in …

    Promoter

    CMV

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Src(KD/YF)-SsrA(Y7I)-mCer3

    Plasmid

    #221000

    Purpose

    this is microTag (Y7I mutation in SsrA)-Src(D388R/Y529F) with mCerulean3 at C-terminal

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA, mCerulean3 (Src Mouse)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    D388R/Y529F in Src, Y7I in SsrA

    Promoter

    pCMV

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SpRYc

    Plasmid

    #175575

    Purpose

    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

    Insert

    SpRYc
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    PID of SpRY grafted onto the N-terminal domain of…

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TRUPATH Triple (bidirectional) GasS

    Plasmid

    #196055

    Purpose

    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AXBEv2

    Plasmid

    #204606

    Purpose

    Mammalian expression of evolved adenine transversion base editor AXBEv2

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ACBE

    Plasmid

    #204607

    Purpose

    Mammalian expression of adenine-to-cytosine base editor ACBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AXBE

    Plasmid

    #204605

    Purpose

    Mammalian expression of adenine transversion base editor AXBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    S. pyogenes Cas9 (D10A)

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge