We narrowed to 7,632 results for: ALP
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Plasmid#180610PurposeBacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129DepositorInsertCALPAIN-7
TagsHIS-GSTExpressionBacterialMutationResidues 1-65Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CALPAIN-7 residues 1-165
Plasmid#180611PurposeBacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25DepositorInsertCALPAIN-7
TagsHIS-SUMOExpressionBacterialMutationResidues 1-165PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS238D1::sptse5-CT_phoA-lacZalpha
Plasmid#192961PurposeTest the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putidaDepositorInsertsptse5-CT_phoA-lacZ_
TagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1317) fused to phoA-lacZal…Available SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
GABA(A)R, Alpha4 scFv [N398A/34]
Plasmid#190551PurposeMammalian Expression of GABA(A)R, Alpha4 scFV. Derived from hybridoma N398A/34.DepositorInsertGABA(A)R, Alpha4 (Rattus norvegicus) recombinant scFV (Gabra4 Mouse)
TagsHA, Sortase, 6xHisExpressionMammalianPromoterCMVAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
GABA(A)R, Alpha2 scFv [N399/19]
Plasmid#190552PurposeMammalian Expression of GABA(A)R, Alpha2 scFV. Derived from hybridoma N399/19.DepositorInsertGABA(A)R, Alpha2 (Rattus norvegicus) recombinant scFV (Gabra2 Mouse)
TagsHA, Sortase, 6xHisExpressionMammalianPromoterCMVAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-PGL-1-mHP1alpha
Plasmid#181894PurposeSoluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mouse, Mustard Weed)
TagsPHR-mCherryExpressionMammalianPromoterCMVAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-nE-mHP1alpha-delCTE
Plasmid#181891PurposeSoluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mouse, Mustard Weed)
TagsPHR-mCherryExpressionMammalianMutationS11E, S12E, S13E, S14E, 14 aa C-terminal deletionPromoterCMVAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRGG-GFP-RGG-mHP1alpha
Plasmid#181898PurposeFluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1DepositorAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD1024 EF1alpha TetON3G in TU5
Plasmid#139261PurposeExpresses TetON3G under hEF1alpha in TUPV5DepositorInsertTetON3G
UseSynthetic BiologyExpressionMammalianPromoterhEF1alphaAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM469 EF1alpha TetON3G in TU4
Plasmid#139260PurposeExpresses TetON3G under hEF1alpha in TUPV4DepositorInsertTetON3G
UseSynthetic BiologyExpressionMammalianPromoterhEF1alphaAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM422 EF1alpha TetON3G in TU3
Plasmid#139259PurposeExpresses TetON3G under hEF1alpha in TUPV3DepositorInsertTetON3G
UseSynthetic BiologyExpressionMammalianPromoterhEF1alphaAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha2 clone2
Plasmid#162123PurposeLentiviral sgRNA plasmid targeting human AMPK alpha2DepositorInsertsgAMPK alpha2 (PRKAA2 Human)
UseLentiviralAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha2 clone3
Plasmid#162124PurposeLentiviral sgRNA plasmid targeting human AMPK alpha2DepositorInsertsgAMPK alpha2 (PRKAA2 Human)
UseLentiviralAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-2alpha T406E
Plasmid#163379PurposeExpress HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr406Glu) plasmidDepositorInsertHypoxia Inducible Factor 2 alpha (EPAS1 Human)
TagsHA- and Clover-taggedExpressionMammalianMutationT406EPromoterCMV PromoterAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-2alpha T406A
Plasmid#163378PurposeExpress HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr406Ala) plasmidDepositorInsertHypoxia Inducible Factor 2 alpha (EPAS1 Human)
TagsHA- and Clover-taggedExpressionMammalianMutationT406APromoterCMV PromoterAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-2alpha S581D
Plasmid#163377PurposeExpress HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser581Asp) plasmidDepositorInsertHypoxia Inducible Factor 2 alpha (EPAS1 Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS581DPromoterCMV PromoterAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-2alpha S581A
Plasmid#163376PurposeExpress HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser581Ala) plasmidDepositorInsertHypoxia Inducible Factor 2 alpha (EPAS1 Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS581APromoterCMV PromoterAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-2alpha T528E
Plasmid#163375PurposeExpress HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr528Glu) plasmidDepositorInsertHypoxia Inducible Factor 2 alpha (EPAS1 Human)
TagsHA- and Clover-taggedExpressionMammalianMutationT528EPromoterCMV PromoterAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-2alpha T528A
Plasmid#163374PurposeExpress HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr528Ala) plasmidDepositorInsertHypoxia Inducible Factor 2 alpha (EPAS1 Human)
TagsHA- and Clover-taggedExpressionMammalianMutationT528APromoterCMV PromoterAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-2alpha S345D
Plasmid#163373PurposeExpress HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser345Asp) plasmidDepositorInsertHypoxia Inducible Factor 2 alpha (EPAS1 Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS345DPromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-2alpha S345A
Plasmid#163372PurposeExpress HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser345Ala) plasmidDepositorInsertHypoxia Inducible Factor 2 alpha (EPAS1 Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS345APromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha S31D
Plasmid#163370PurposeExpress HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Asp) plasmidDepositorInsertHypoxia Inducible Factor 1 alpha S31D (HIF1A Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS31DPromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha S31G
Plasmid#163369PurposeExpress HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Gly) plasmidDepositorInsertHypoxia Inducible Factor 1 alpha S31G (HIF1A Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS31GPromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha S786D
Plasmid#163368PurposeExpress HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser786Asp) plasmidDepositorInsertHypoxia Inducible Factor 1 alpha S786D (HIF1A Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS786DPromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha S786A
Plasmid#163367PurposeExpress HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser786Ala) plasmidDepositorInsertHypoxia Inducible Factor 1 alpha S786A (HIF1A Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS786APromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha2 P35S:RDF:T35S (GB1508)
Plasmid#160613PurposeTU for the constitutive expression of Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)DepositorInsertRDF
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)
Plasmid#160614PurposeTU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.DepositorInsertPhiC31
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromotermini35sAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)
Plasmid#160615PurposeTU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.DepositorInsertPhiC31
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromotermini35sAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-C2X -MTalpha-FliG
Plasmid#154361PurposeBacterial expression of MtalphaDepositorInsertMtalpha
ExpressionBacterialAvailable SinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE (hygro) hYAP 1-1alpha
Plasmid#124147PurposeProtein expression for funtional studies of cell growth promotionDepositorAvailable SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE (hygro) hYAP 1-2alpha
Plasmid#124151PurposeProtein expression for funtional studies of cell growth promotionDepositorAvailable SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS puro miR30-TAK1
Plasmid#117162PurposeTarget site AGCGCCCTTCAATGGAGGAAATDepositorInsertshRNA targeting miR30
UseLentiviralAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS puro miR30-STAT2
Plasmid#117161PurposeTarget site TTTAAGTTCCACAGACTTGGADepositorInsertshRNA targeting miR30
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H
Plasmid#108374PurposeExpresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertExpressionMammalianPromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Alpha-Actinin-19
Plasmid#57782PurposeLocalization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528DepositorAvailable SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-CFP
Plasmid#50482Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsCFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the CFP open…PromoterCMVAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-alpha4-SEP
Plasmid#45607DepositorInsertnAChRAlpha4-SEP
TagsSEP- TagExpressionMammalianPromoterCMVAvailable SinceAug. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha D291N mutant
Plasmid#128813PurposeTo make virus expressing Doc2alpha D291 mutantDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsIRES-EGFPMutationD291NPromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha
Plasmid#179718PurposeLentiviral expression of synaptic vesicle-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD
Plasmid#179722PurposeLentiviral expression of lysosome-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralAvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-VP64-dCas9-VP64_Blast
Plasmid#192650Purpose3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance markerDepositorInsertVP64-dCas9-VP64
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-alpha
Plasmid#88846PurposeCRISPR KO of Trp53DepositorAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 Alpha Spike
Plasmid#185691PurposeExpresses codon-optimized full length SARS-CoV-2 Alpha SpikeDepositorInsertSARS-CoV-2 Alpha Spike (S )
ExpressionMammalianMutationContains the following mutations: Δ69-70, Δ145, N…Promoterchicken β-actin promoter, CMV enhancerAvailable SinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
Plasmid#71396Purpose2Gi2R assayDepositorInserts2xGFP:NES
2xRFP:NLS
UseLentiviralTagsIRESExpressionMammalianPromoterEF1alpha and IRESAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSV human p85 alpha (HA tag)
Plasmid#11499DepositorAvailable SinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 GFP B55 alpha shRNA 3-1
Plasmid#10679DepositorAvailable SinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter 2kb luciferase
Plasmid#8887DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
Anti-GABA(A)R, Alpha1 [N95/35R]
Plasmid#177574PurposeMammalian Expression Plasmid of anti-GABA(A)R, Alpha1 (Human). Derived from hybridoma N95/35.DepositorInsertanti-GABA(A)R, Alpha1 (Homo sapiens) recombinant mouse monoclonal antibody (GABRA1 Mouse)
ExpressionMammalianPromoterDual CMVAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Slo1/BKAlpha maxi-K+ channel scFv [L6/60]
Plasmid#199421PurposeMammalian expression of Slo1/BKAlpha maxi-K+ channel scFv. Derived from hybridoma L6/60.DepositorInsertrecombinant mouse scFv targetingSlo1/BKAlpha maxi-K+ channel (Rat) (Kcnma1 Mouse)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha P402A/P564A-pBabe-puro
Plasmid#19005DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 402 to Alanin…Available SinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only