We narrowed to 30,406 results for: Ide

1. p3E-U6a-U6c-uqcrc1 guide

   Plasmid

   #107602

   Purpose

   An entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   uqcrc1 guide RNA sequences
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. RalA (G23V)(mature peptide)-pcw107-V5

   Plasmid

   #64643

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   RALA (RALA Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   G23V

   Promoter

   PGK

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

3. RalA (G23V)(mature peptide)-pcw107

   Plasmid

   #64642

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   RALA (RALA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G23V

   Promoter

   PGK

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pC016 - LwCas13a guide expression backbone with U6 promoter

   Plasmid

   #91906

   Purpose

   Backbone for cloning LwCas13a guides under U6 promoter

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Has Service

   Cloning Grade DNA

   Insert

   LwCas13a Guide site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

   Plasmid

   #99248

   Purpose

   single-vector system with EGFP reporter for use in CROPseq knockout screens

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. single polypeptide FPX biosensor for calcium ion

   Plasmid

   #60887

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for calcium ion
   

   Use

   Tags

   HindIII site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2-LyzC-RacFRET-U6ac-ctrl guides

   Plasmid

   #168247

   Purpose

   "label active Rac; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   RacFRET
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

   Plasmid

   #186993

   Purpose

   AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-s1006-gggs-d122, non-targeting crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   s1006-gggs-d1221 deletion

   Promoter

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

   Plasmid

   #186991

   Purpose

   AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-r1045-gggs-r1122, non-targeting crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   r1045-gggs-r1122 deletion

   Promoter

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

    Plasmid

    #157986

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. single polypeptide FPX biosensor for both caspase-3 and -8

    Plasmid

    #60886

    Purpose

    FPX biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-3 and 8
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Tol2-LyzC-GFP-U6ac-ctrl-guides

    Plasmid

    #168240

    Purpose

    "neutrophil specific GFP with ubiquitous ctrl sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    control sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Tol2-LyzC-GFP-U6ac-rac2-guides

    Plasmid

    #168241

    Purpose

    "neutrophil specific GFP with ubiquitous rac2 sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    rac2 sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

    Plasmid

    #157984

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Tol2-LyzC-GFP-U6ac-cdk2-guides

    Plasmid

    #168250

    Purpose

    "neutrophil specific GFP with ubiquitous cdk2 sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    cdk2 sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Tol2-LyzC-RacFRET-U6ac-rac2 guides

    Plasmid

    #168248

    Purpose

    "label active Rac under Rac2 neutrophil-specific knockout background"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    RacFRET
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    LyzC

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCF821-sgCIDE-10_U6-sgRNA-EF1a-mNeonGreen

    Plasmid

    #211670

    Purpose

    U6-sgRNA-EF1a-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-10 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCF821-sgCIDE-5_U6-sgRNA-EF1a-mNeonGreen

    Plasmid

    #211665

    Purpose

    U6-sgRNA-EF1a-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-5 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCF821-sgCIDE-6_U6-sgRNA-EF1a-mNeonGreen

    Plasmid

    #211666

    Purpose

    U6-sgRNA-EF1a-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-6 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCF821-sgCIDE-7_U6-sgRNA-EF1a-mNeonGreen

    Plasmid

    #211667

    Purpose

    U6-sgRNA-EF1a-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-7 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge