We narrowed to 7,125 results for: RAP

1. TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA

   Plasmid

   #201965

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Use

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   E198A, R199A,E200A, D201A

   Promoter

   Available since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA

   Plasmid

   #201967

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Use

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   R232A, K233A, R234A, K235A, R236A

   Promoter

   Available since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-luciferase-reporter-CDK5RAP2-SVA

   Plasmid

   #202828

   Purpose

   pGL3 luciferase reporter with CDK5RAP2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   intronic SVA within CDK5RAP2 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A

   Plasmid

   #175752

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50A

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   GABARAPL2 (GABARAPL2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Y49A L50A

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-PGK-RAP2A-V12-puro

   Plasmid

   #156171

   Purpose

   Lentiviral vector for expression of RAP2A-V12, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Valine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-PGK-RAP2A-N17-puro

   Plasmid

   #156172

   Purpose

   Lentiviral vector for expression of RAP2A-N17, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-CMV GFP-GABARAPL1 G116

   Plasmid

   #123119

   Purpose

   Expresses EGFP-GABARAPL1 G116 in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deleted amino acid 117. Stop codon after G116

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRAP-COMP-blac-flag-his

   Plasmid

   #111005

   Purpose

   Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

   Depositor

   Gavin Wright

   Article

   Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

   Insert

   thrombospondin-related anonymous protein (TRAP) (TRAP Synthetic)
   

   Use

   Tags

   Exogenous signal peptide of mouse origin and rat …

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-FLAG-WRAP53beta-siRNA-resistant

   Plasmid

   #64680

   Purpose

   expresses siRNA resistant WRAP53beta

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   WD40-encoding RNA antisense to P53 (WRAP53 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   siRNA resistant

   Promoter

   CMV

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pMT Draper-delta ITAM-GFP

    Plasmid

    #126655

    Purpose

    Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper with ITAM Tyr residues mutated to Ala
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Cytoplasmic ITAM Tyr residues mutated to Phe

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT FRB-Draper INT-GFP

    Plasmid

    #126665

    Purpose

    Inducible expression of a synthetic FRB-Draper receptor in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    FRB-Draper INT domain
    

    Use

    Tags

    Expression

    Insect

    Mutation

    K986N- see depositor comments below

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT FRB-Draper INT-mCherry

    Plasmid

    #126666

    Purpose

    Inducible expression of a synthetic FRB-Draper receptor in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    FRB-Draper INT domain mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET His10-Draper delta ITAM

    Plasmid

    #126671

    Purpose

    Expression of His10-Draper delta ITAM mutant for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper cytoplasmic ITAM residues mutated to Phe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cytoplasmic ITAM residues mutated to Phe

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT311 AAV-Rapamycin Inducible-NZF-Citrine

    Plasmid

    #202060

    Purpose

    Inducible AAV activator

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-NZF-Citrine
    

    Use

    AAV; Aav with reporter

    Tags

    Expression

    Mammalian

    Mutation

    ITR deletion

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

    Plasmid

    #187578

    Purpose

    Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    WRAP53 (WRAP53 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

    Plasmid

    #202824

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 - two

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD649-HAsp-IL1RAPL1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156778

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    IL1RAPL1 (IL1RAPL1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2

    Plasmid

    #185449

    Purpose

    Coexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV site, and human TRF2 with a zz tag and TEV site

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Inserts

    TRF2 (TERF2 Human)
    RAP1 (TERF2IP Human)
    

    Use

    Tags

    MBP, YBBR, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti PGK Puro GFP-GABARAPL1 G116

    Plasmid

    #123243

    Purpose

    Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Promoter

    PGK

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pD649-HAsp-IL1RAP-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156790

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    IL1RAP (IL1RAP Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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