We narrowed to 2,298 results for: SE

Showing: 361 - 380 of 2298 results

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FLAG-MBP1-K295TAG-W340A-S233H-T237H.pcDNA3-k

Plasmid

#127400

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon, W340A,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag VP64 gRNA17 (FWA)

Plasmid

#120250

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGC681

Plasmid

#107019

Purpose

GFP reporter of lag-2 driven by 405 bp of promoter

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Tags

GFP

Expression

Worm

Promoter

lag-2

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGC643

Plasmid

#107016

Purpose

GFP reporter of lag-2 driven by 0.5 kb promoter

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Tags

GFP

Expression

Worm

Promoter

lag-2

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-RFP-miR-30 backbone

Plasmid

#97147

Purpose

As a control of miRNA expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAM5084

Plasmid

#85105

Purpose

Ptrc-ECFP-KaiC fusion cloned into Km resistance gene of pACYC177. To look for KaiC localization in E. coli.

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

ecfp-KaiC

Tags

ECFP

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJK040 (pET-PurQ-A128T)

Plasmid

#73840

Purpose

Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

Depositor

T. J. Kappock, JoAnne Stubbe

Article

Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Expression

Bacterial

Mutation

A128T mutant

Promoter

T7

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Slc25a15 mod

Plasmid

#48161

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Slc25a15

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Slc25a15

Plasmid

#48160

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Slc25a15

Use

Luciferase

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
GFP-N-WASP

Plasmid

#47406

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

N-WASP

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMP71-Flt3L

Plasmid

#174600

Purpose

Secretes the extracellular domain of murine Flt3L

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

secretes extracellular domain of mouse Flt3-L

Expression

Mammalian

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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mNG2(1-10)_tdTomato

Plasmid

#184169

Purpose

Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(1-10)
tdTomato

Use

Lentiviral

Expression

Mammalian

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSimple-miR302/367

Plasmid

#98748

Purpose

Episomal reprogramming

Depositor

Sara Howden, James Thomson

Article

Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

Insert

miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2B -1538/+64

Plasmid

#24942

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pLYS1-FLAG-MitoGFP-HA

Plasmid

#50057

Purpose

Expression of GFP targeted to mitochondria with Flag and HA tags

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

Mito-GFP

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PB-NES-ZapCV2-Zinc dead

Plasmid

#203850

Purpose

Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

Depositor

Amy Palmer

Article

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2-Ala zinc dead sensor

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

Cys and His in Zap1 is mutated with Ala

Promoter

CMV promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta K85A pcDNA3

Plasmid

#14755

Depositor

Jim Woodgett

Article

Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Insert

GSK3 beta K85A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

K85A. Kinase dead mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-FAH-rtTA3G

Plasmid

#120309

Purpose

An AAV vector that expresses rtTA3G under the c-fos promoter

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

rtTA3G

Use

AAV

Promoter

c-fos

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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OSCA2.3

Plasmid

#136595

Purpose

Expresses the mechanosensitive channel OSCA2.3 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA2.3 (HYP1 Mustard Weed)

Tags

pIRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1119 (pDonor)

Plasmid

#160732

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA (Mini-Tn)

Expression

Bacterial

Promoter

N/A

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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