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pRSET-His-APEX2

Plasmid

#201471

Purpose

Recombinant soluble APEX2 for SLAPSHOT labeling

Depositor

Yuh-Nung Jan

Article

Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

Insert

APEX2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG mito-mTagBFP2

Plasmid

#105011

Purpose

Mitochondrial marker

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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10TALE_Pmin_fLuc

Plasmid

#160663

Purpose

Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain

Depositor

Roman Jerala

Article

Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

Insert

10TALE_Pmin_fLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

minimal

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Human NCKX4-AS-long (pcDNA3.1+)

Plasmid

#75211

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Use

Tags

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-M13-TEV-C-P2A-TdTomato

Plasmid

#92389

Purpose

expresses Cal-light M13-TEV-C component and TdTomato in mammalian cells

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Use

Tags

TdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR - CaSR-EGFP

Plasmid

#191503

Purpose

A zebrafish CaSR-EGFP construct used for Gateway cloning.

Depositor

Michael Granato

Article

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

none

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-6P-SEW-YC3.6

Plasmid

#31379

Depositor

Fritjof Helmchen

Article

Minderer et al J Physiol. 2011 Nov 14.

Insert

Yellow Cameleon 3.60

Use

AAV

Tags

Venus and eCFP

Expression

Mutation

Promoter

Available since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PSD95-GCaMP2

Plasmid

#18931

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

PSD95-GCaMP2 (Dlg4 Rat)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pENTR-D-TOPO-kcnn2

Plasmid

#164958

Purpose

Zebrafish kcnn2 gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn2 (kcnn2 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TO-DsRed

Plasmid

#16340

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

DsRed

Use

Tags

Expression

Mammalian

Mutation

5'Prime Site: Not I 3'Primer Site : No…

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
TroponinT-Gateway

Plasmid

#46026

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Type

Empty backbone

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

TNNT2

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BREP

Plasmid

#172337

Purpose

BRET-Based Bioluminescent Red Protein

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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hME-pOX

Plasmid

#178181

Purpose

Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

Depositor

Ming-Feng Tsai

Article

Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

Insert

human MCU-EMRE fusion protein

Use

Tags

1D4

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Golgi-DKAR

Plasmid

#49790

Purpose

FRET-based, Golgi-targeted kinase activity reporter for PKD

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

Insert

D kinase activity reporter

Use

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
PCx35IL-pET42

Plasmid

#42916

Depositor

John O'Brien

Article

Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

Insert

Connexin 35 cytoplasmic loop

Use

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

cytoplasmic loop is aa 101-179

Promoter

T7

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX4-AS-long (pcDNA3.1+)

Plasmid

#75208

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (Slc24a4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-hCD4-GCaMP2

Plasmid

#18932

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

hCD4-GCaMP2 (CD4 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pENTR-D-TOPO-kcnn1a

Plasmid

#164956

Purpose

Zebrafish kcnn1a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn1a (kcnn1a Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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FuPick-shRNA

Plasmid

#31614

Depositor

Robert Malenka

Article

Citri et al J Neurosci. 2010 Dec 8. 30(49):16437-52.

Insert

PICK1 shRNA (Pick1 Rat)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pLJM1-NtermRGS2-GFP-CIBN

Plasmid

#112258

Purpose

Expresses alpha-helix component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

NtermRGS2-GFP-CIBN

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge