Skip to main content
Addgene
Showing: 361 - 380 of 15672 results

  4. MKC86_HBA1(tEPOR-2A-YFP)

    Plasmid
    #232405
    Purpose
    AAV production plasmid for tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at HBA1 locus using HBA1-sg4 gRNA. HBA1 UTRs flank full tEPOR-2A-YFP cassette. Homology arms mediate full HBA1 replacement.
    Depositor
    Kyle Cromer, Matthew Porteus
    Article
    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552
    Inserts
    Truncated Erythropoietin Receptor (EPOR Human)
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232405-plasmid-map-sequence-id-462759

    Enlarge

  7. MKC177_CCR5(PGK-synEPOR)

    Plasmid
    #232415
    Purpose
    AAV production plasmid for PGK(synEPOR) vector from Figs. 3-5 that mediates HDR at CCR5 locus using CCR5 gRNA. synEPOR is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.
    Depositor
    Kyle Cromer
    Article
    Shah et al bioRxiv 2024.04.12.589260
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232415-plasmid-map-sequence-id-465035

    Enlarge

  11. pFsynW SYT1deltaC2AB reporter

    Plasmid
    #197279
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197279-plasmid-map-sequence-id-389097

    Enlarge

  12. pB6-Rosa26-cre_dre_reporter_1_2

    Plasmid
    #185966
    Purpose
    B6J Rosa26-cre dre reporter 1.2 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185966-plasmid-map-sequence-id-367346

    Enlarge

  13. pB6-Rosa26-cre_dre_reporter_1_1b

    Plasmid
    #185965
    Purpose
    B6J Rosa26-cre dre reporter 1.1b allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185965-plasmid-map-sequence-id-367345

    Enlarge

  14. pB6-Rosa26-cre_dre_reporter_1_1a

    Plasmid
    #185964
    Purpose
    B6J Rosa26-cre dre reporter 1.1a allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185964-plasmid-map-sequence-id-367344

    Enlarge

  15. pB6-Rosa26-cre_dre_reporter_1_1

    Plasmid
    #185963
    Purpose
    B6J Rosa26-cre dre reporter 1.1 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    EGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185963-plasmid-map-sequence-id-367343

    Enlarge

  16. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid
    #182678
    Purpose
    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Mouse, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182678-plasmid-map-sequence-id-363084

    Enlarge

  17. pORANGE_NFL linker-3xFLAG KI

    Plasmid
    #182679
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182679-plasmid-map-sequence-id-363017

    Enlarge

Showing: 361 - 380 of 15672 results