We narrowed to 78,806 results for: TRI
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Plasmid#216418PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.DepositorInsertdmito-oROS-HT
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-δWT-nAchR
Plasmid#97188PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertDelta nicotinic acetylcholine receptor (Chrnd Mouse)
UseTagsExpressionBacterialMutationWTPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-γWT-nAchR
Plasmid#97187PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertGamma nicotinic acetylcholine receptor (Chrng Mouse)
UseTagsExpressionBacterialMutationWTPromoterT7Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FOXM1b
Plasmid#68811PurposeDoxycycline inducible lentiviral vector for FOXM1b expression in mammalian cellsDepositorInsertFOXM1b (FOXM1 Human)
UseLentiviral; Doxycycline inducibleTagsHAExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Venus-cpVenus-FLARE-Cameleon
Plasmid#123350PurposeYellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.DepositorInsertVenus-cpVenus-FLARE-Cameleon
UseTags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-GRAM1b
Plasmid#211494PurposeExpression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cellsDepositorInsertGRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-ICUE
Plasmid#123355PurposeYellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.DepositorInsertVenus-cpVenus-FLARE-ICUE
UseTags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC059-HisMS2_PLP_NucPhos_pac
Plasmid#155039PurposeGeneration of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein geneDepositorInsertsMaturation Protein
Coat Protein Dimer
Nucleocapsid Phosphoprotein
UseTagsExpressionBacterialMutationRemove TypeIIs Restriction SitesPromoterT7Available sinceSept. 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-Venus-cpVenus-FLARE-AKAR
Plasmid#123329PurposeYellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.DepositorInsertVenus-cpVenus-FLARE-AKAR
UseTags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)
Plasmid#123330PurposeNegative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.DepositorInsertVenus-cpVenus-FLARE-AKAR(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-ChRmine-mScarlet
Plasmid#137158PurposeViral expression of ChRmine-p2a-mScarletDepositorHas ServiceAAV8InsertChRmine-p2a-mScarlet
UseAAVTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FOXM1c
Plasmid#68810PurposeDoxycycline inducible lentiviral vector for FOXM1c expression in mammalian cellsDepositorInsertFOXM1c (FOXM1 Human)
UseLentiviral; Doxycycline inducibleTagsFLAGExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP WT
Plasmid#197575PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replicationDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-Tom20-mEGFP-reHaloTagF
Plasmid#200190PurposeTom20 with reversible HaloTagF variant for fluorescent labelingDepositorInsertTom20 (TOMM20 Human)
UseTagsmEGFP and reHaloTagFExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/38]
Plasmid#182095PurposeMammalian Expression of GFP scFv. Derived from hybridoma N86/38.DepositorInsertrecombinant mouse scFv targeting GFP
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX NPC1-FLAG-TiD
Plasmid#199748PurposeLentiviral construct to stably express NPC1 TurboIDDepositorInsertNPC1 (NPC1 Human)
UseTagsFlag and TurboIDExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 flag-cFLIP
Plasmid#211529PurposeExpresses flag-tagged cFLIP (CFLAR)DepositorInsertcFLIP (CFLAR Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-loxp Kir2.1-2A-GFP
Plasmid#161574PurposeExpression of Kir2.1-2A-GFP in a Cre-dependent fashionDepositorInsertKir2.1-2A-GFP (Kcnj2 Mouse)
UseAAVTagsExpressionMutationNAPromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tau (wt) -VC
Plasmid#87369Purposeexpresses hTau fused to the C-terminal part of VenusDepositorInsertMAPT (MAPT Human)
UseTagsc-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
VN-Tau (wt)
Plasmid#87368Purposeexpresses hTau fused to the N-terminal part of VenusDepositorInsertMAPT (MAPT Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBK- Ma PylRS WT
Plasmid#197573PurposeExpresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS WT
UseTagsExpressionBacterialMutationPromoterGlnS (constitutive)Available sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-TM-VC
Plasmid#135499PurposeMammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TMDepositorInsertTAPBPR (TAPBPL Human)
UseTagsLuminal/Extracellular FLAG epitope tag, Signal pe…ExpressionMammalianMutationswitched transmembrane domain with that of MHC-IPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc
Plasmid#113862PurposeTriple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.DepositorInsertsCMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2
Plasmid#166013Purposemammalian expression of MERS-CoV soluble spike trimer proteinDepositorInsertMERS-CoV S (spike)
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-iRFP670
Plasmid#99377PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-iRFP670
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Cep170 (Nigg PID200)
Plasmid#41150DepositorInsertCep170 (CEP170 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMV, T7Available sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-PM
Plasmid#164784PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
UseTagsNanoLucExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M87-303: CMV51p> SARS-CoV S-2P-T4f-3C-His8-Strep2x2
Plasmid#166012Purposemammalian expression of SARS-CoV soluble spike trimer proteinDepositorInsertSARS-CoV S (spike)
UseTags3C-His8-Strep2x2ExpressionMammalianMutationPromoterCMV51Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only