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pcDNA3.1/CALR wtΔKDEL YD/FL

Plasmid

#214805

Purpose

Mammalian expression of human CALR wtΔKDEL YD/FL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR wtΔKDEL YD/FL (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1/CALR ins5-His

Plasmid

#214796

Purpose

Baculovirus expression of human CALR ins5

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR ins5 (CALR Human)

Tags

His

Expression

Insect

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wt

Plasmid

#214797

Purpose

Mammalian expression of human CALR wt

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR wt (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR del52

Plasmid

#214798

Purpose

Mammalian expression of human CALR del52

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR del52 (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5

Plasmid

#214799

Purpose

Mammalian expression of human CALR ins5

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR ins5 (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR WTΔKDEL

Plasmid

#214790

Purpose

Mammalian expression of human CALR wtΔKDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR wtΔKDEL (CALR Human)

Tags

non

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Lrp5 gRNA

Plasmid

#246548

Purpose

CRISPR vector co-expressing Cas9 and a mouse Lrp5 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Lrp5 gRNA (Lrp5 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Itgb1 gRNA

Plasmid

#246577

Purpose

CRISPR vector co-expressing Cas9 and a mouse Itgb1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Itgb1 gRNA (Itgb1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Insr gRNA

Plasmid

#246570

Purpose

CRISPR vector co-expressing Cas9 and a mouse Insr gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Insr gRNA (Insr Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Lrp6 gRNA

Plasmid

#246549

Purpose

CRISPR vector co-expressing Cas9 and a mouse Lrp6 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Lrp6 gRNA (Lrp6 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Itga5 gRNA

Plasmid

#246573

Purpose

CRISPR vector co-expressing Cas9 and a mouse Itga5 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Itga5 gRNA (Itga5 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mPkm2-T328E

Plasmid

#242814

Purpose

Lentiviral vector expressing mouse Pkm2-T328E mutant tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

pyruvate kinase, muscle (Pkm Mouse)

Use

Lentiviral

Tags

EGFP

Mutation

T328E

Promoter

Ubiquitin C

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mPkm2-T328A

Plasmid

#242813

Purpose

Lentiviral vector expressing mouse Pkm2-T328A mutant tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

pyruvate kinase, muscle (Pkm Mouse)

Use

Lentiviral

Tags

EGFP

Mutation

T328A

Promoter

Ubiquitin C

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-Rubicon-CGHL

Plasmid

#221658

Purpose

Rubicon mutated at four residues in the RH domain to disrupt Rab7 binding, tagged at the N-terminus with mCherry

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

RUBCN (RUBCN Human)

Tags

mCherry

Expression

Mammalian

Mutation

C912G, C915G, H920L, C923G

Promoter

CMV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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L4869 hVEGFA-121 in pcDNA backbone

Plasmid

#244180

Purpose

Constitutive expression of human VEGFA-121 isoform

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

VEGFA-121 (VEGFA Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET24+ CUL5(1-384)

Plasmid

#246504

Purpose

Recombinant protein expression of CUL5(1-384)

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

cullin-5 (CUL5 Human)

Expression

Bacterial

Mutation

Deleted amino acids 385-780

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gna15

Plasmid

#246003

Purpose

Express HA N-terminal tagged mouse Galpha15 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gna15 (Gna15 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSG5-CAGEc-PRKACA (Nrdj1)

Plasmid

#241421

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product DNAJ-PKAc, Figure 4, Extended Figure 8-9 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

CAGEc-PRKACA (Nrdj1) (PRKACA Synthetic, Human)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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L4868 hVEGFA-165 in pcDNA backbone

Plasmid

#244179

Purpose

Constitutive expression of human VEGFA-165 isoform

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

VEGFA-165 (VEGFA Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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L4300 hIL10 in pcDNA backbone

Plasmid

#244185

Purpose

Constitutive expression of human IL-10 (with its signal sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

IL-10 (IL10 Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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