We narrowed to 4,490 results for: Gca

Showing: 3781 - 3800 of 4490 results

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B2M-SDMutation-gRNA

Plasmid

#215547

Purpose

gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

B2M (B2M Human)

Use

CRISPR

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-KRAB_sgSTK3i_#1 (PuroR)

Plasmid

#209771

Purpose

CRISPRi for STK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

dCas9-KRAB, sgSTK3i (STK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mPHB2-2

Plasmid

#198484

Purpose

lentiviral stable expression of mPHB2 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mPhb2 (Phb2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Wnk1

Plasmid

#174874

Purpose

CRISPR vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Wnk1 (Wnk1 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform2_untagged-Puro

Plasmid

#192002

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2 (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMVTRE3G

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform1_I142V-Puro

Plasmid

#192004

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform1 I142V mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform1 I142V (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMVTRE3G

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform1_R45W-Puro

Plasmid

#192003

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform1 R45W mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform1 R45W (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMVTRE3G

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-tdTomato166/892

Plasmid

#188488

Purpose

gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Flag-Mcm2 targeting sgRNA

Plasmid

#186937

Purpose

Genomic targeting of Flag tag at Mcm2 N-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Flag-Mcm2 targeting sgRNA (Mcm2 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE_NFL linker(A6TAG)-3xFLAG KI

Plasmid

#182680

Purpose

Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)

Expression

Mammalian

Promoter

U6 and chicken beta-actin promoter

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Gpi1

Plasmid

#175934

Purpose

knockout mouse Gpi1

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Gpi1 gRNA (Gpi1 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Kmt2b-4

Plasmid

#162538

Purpose

sgRNA targeting Kmt2b

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Kmt2b (Kmt2b Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Alb-3

Plasmid

#162546

Purpose

sgRNA targeting Alb

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Alb (Alb Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPN431

Plasmid

#137869

Purpose

Expression of gRNA a2 targeting TCF4 for CRISPRa; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPN446

Plasmid

#137868

Purpose

Expression of gRNA a1 targeting TCF4 for CRISPRa; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-W

Plasmid

#105031

Purpose

Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Ndufa2 (Ndufa2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

Plasmid

#128758

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #3 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 C2CD4A-TSS-guide4

Plasmid

#125483

Purpose

CRISPR-mediated activation of C2CD4A. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 C2CD4A/B-enh3-guide2

Plasmid

#125474

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 C2CD4A/B-enh3-guide1

Plasmid

#125473

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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