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pJK340

Plasmid

#71837

Purpose

Cloned region contains Acetobacter aceti 1023 purE(H59N) gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine

Promoter

lac

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK346

Plasmid

#71836

Purpose

Cloned region contains Acetobacter aceti 1023 purE(H59D) gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to aspartate

Promoter

lac

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-G

Plasmid

#59798

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele G

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele G

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-F

Plasmid

#59797

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele F

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele F

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-D

Plasmid

#59795

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele D

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele D

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-B

Plasmid

#59793

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele B

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele B

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pME18S-12053

Plasmid

#55603

Purpose

Aedes aegypti NPY-like receptor AAEL012053 (NPYLR3) full-length clone in mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

NPYLR3

Expression

Mammalian

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pA9HD

Plasmid

#8588

Depositor

Cynthia Wolberger

Article

LaRonde-LeBlanc et al Genes Dev 2003 Aug 15;17(16):2060-72.

Insert

HoxA9 190-271with hexapeptide (HOXA9 Human)

Tags

His

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pAJ03

Plasmid

#14910

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Tags

Ss era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAJ04

Plasmid

#14911

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Tags

Ss era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-B1-B2-HIS-ybbr

Plasmid

#117979

Purpose

SdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_N2N3-B1-B2

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-B1-HIS-ybbr

Plasmid

#117980

Purpose

SdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_N2N3-B1

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 2

Plasmid

#128571

Purpose

for cloning identified individual SPECS

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9_sgRNA_expression_in_pBluescript

Plasmid

#122089

Purpose

U6 driven SpCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MCS-BirA(R118G)-HA

Plasmid

#36047

Purpose

To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA(R118G)-HA

Tags

HA

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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pOVC1_RsLP-LOV_X

Plasmid

#131177

Purpose

Expresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

RsLP-LOV

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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SpyTag003-MBP

Plasmid

#133450

Purpose

Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to N-terminus of MBP via a GSGE…

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD

Expression

Mammalian

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pICH-roGFP2-ORP1

Plasmid

#191677

Purpose

Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

redox(ro)GFP2-ORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

Plasmid

#154303

Purpose

Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Inserts

histone H1
γ-tubulin

Use

Dictyostelium expression

Tags

mEGFP and mRuby3

Promoter

actin15

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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