We narrowed to 22,382 results for: Sis

1. pCold I-Hero13

   Plasmid

   #187929

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero13

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero13 (BEX3 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZLblasti-HA-MEK1 C121S

   Plasmid

   #53165

   Purpose

   Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C121S

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. hHSF1 S303A

   Plasmid

   #118363

   Purpose

   This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   S303A

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-TAP(FLAG-HA) CYLD

   Plasmid

   #60031

   Purpose

   CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Retroviral

   Tags

   FLAG, HA, and IRES Puro

   Expression

   Mammalian

   Mutation

   Promoter

   LTR-driven expression

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

5. CD8-dCherry-FRB

   Plasmid

   #100739

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD8A (CD8A Human)
   

   Use

   Tags

   FRB and dCherry

   Expression

   Mammalian

   Mutation

   Dark mCherry (K70N) non-fluorescent

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMMP-NGFR:LMP1-IRES-mRFP

   Plasmid

   #36974

   Depositor

   Bill Sugden

   Article

   Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

   Insert

   NGFR:LMP1 (NGFR, LMP-1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   NGFR:LMP1 chimeric receptor consists of aa 1–276 …

   Promoter

   CMV

   Available since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKIF1C-2xFLAG

   Plasmid

   #130979

   Purpose

   Expression of KIF1C-2xFLAG in mammalian cells.

   Depositor

   Anne Straube

   Article

   Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi

   Insert

   KIF1C-2xFLAG (KIF1C Human)
   

   Use

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   4 silent mutations that make this construct resis…

   Promoter

   CMV

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEXfrt-SaCas9-U6-sgGria3

   Plasmid

   #124869

   Purpose

   Mutagenesis of Gria3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria3 (Gria3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSUPER-retro-puro-tetO-Mek1-shRNA

   Plasmid

   #53179

   Purpose

   Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (Map2k1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAN19-3xFLAG-TIR1-NOSt

    Plasmid

    #108545

    Purpose

    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Gal4-VP16-HSF1Δ∼PRD

    Plasmid

    #71727

    Purpose

    plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    Gal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Herpes Simplex Virus protein VP16, Budding Yeast, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIDTSmart-CMV-PLRS1

    Plasmid

    #217365

    Purpose

    Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

    Depositor

    Abhishek Chatterjee

    Article

    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

    Insert

    leucyl-tRNA synthetase polyspecific mutant
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M40I, T252A, Y499I, Y527A, H529G

    Promoter

    CMV

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-mEGFP-hCTNNA1_E307K

    Plasmid

    #234560

    Purpose

    CTNNA1 butterfly eye mutant

    Depositor

    Cara Gottardi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

    Insert

    mEGFP:hCTNNA1_E307K (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    E307K

    Promoter

    CMV

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-mEGFP-hCTNNA1_R551A

    Plasmid

    #234558

    Purpose

    CTNNA1 M2-M3 salt-bridge disrupting mutant

    Depositor

    Cara Gottardi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

    Insert

    mEGFP:hCTNNA1_R551A (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    R551A

    Promoter

    CMV

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-mEGFP-hCTNNA1_DM2,3-domain

    Plasmid

    #234556

    Purpose

    CTNNA1 M2,3-domain deletion mutant

    Depositor

    Cara Gottardi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

    Insert

    mEGFP:hCTNNA1_DM2,3-domain (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Deletion of aa 397-509

    Promoter

    CMV

    Available since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-mEGFP-hCTNNA1_DM1-domain

    Plasmid

    #234555

    Purpose

    CTNNA1 M1-domain deletion mutant

    Depositor

    Cara Gottardi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

    Insert

    mEGFP:hCTNNA1_DM1-domain (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Deletion of aa 271-396

    Promoter

    CMV

    Available since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-AtmiR173aTS-B/c

    Plasmid

    #227964

    Purpose

    Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    AtmiR173aTS
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. MT2

    Plasmid

    #223330

    Purpose

    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)
    

    Use

    Tags

    3HA-TurboID-EGFP

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-HA-CBS(opt)-FLAG

    Plasmid

    #230088

    Purpose

    expressing human CBS

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    CBS (CBS Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Multiple wobble mutations

    Promoter

    CMV

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-HA-L2HGDH(opt)-FLAG

    Plasmid

    #230089

    Purpose

    expressing human L2HGDH

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    L2HGDH (L2HGDH Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Multiple wobble mutations

    Promoter

    CMV

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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