We narrowed to 79,973 results for: TRI

1. pWM_12x601_35bpLinker

   Plasmid

   #157790

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_35bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 35S::GeNL/pCAMBIA1301

   Plasmid

   #190070

   Purpose

   Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana

   Depositor

   Takeharu Nagai

   Article

   Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

   Insert

   Green-enhanced Nano-lantern
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLP2

   Plasmid

   #209989

   Purpose

   Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. myc-mTOR (1-1482 aa)

   Plasmid

   #21745

   Depositor

   David Sabatini

   Article

   Kim et al Mol Cell 2003 Apr;11(4):895-904.

   Insert

   mTOR (1-1482) (Mtor Rat)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   amino acids 1-1482

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pCAG_EGFP-GFE3

   Plasmid

   #79869

   Purpose

   Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-GFE3
   

   Use

   Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Beta-actin

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUAP4

   Plasmid

   #136079

   Purpose

   Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. AAV.hSyn.GLPLight-ctr

   Plasmid

   #187468

   Purpose

   Expresses GLPLight-ctr in mammalian cells

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

   Insert

   GLPLight-ctr
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mutation

   Promoter

   human Synapsin-1

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Halo-TCAB1 KI sgRNA

   Plasmid

   #207611

   Purpose

   sgRNA for HaloTag insertion into the endogenous TCAB1 locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   CCAAAGTCTTCATACTGCAG
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. L1_UBE2:mS-Lt-Ck3

   Plasmid

   #136131

   Purpose

   L1 in position 3, mark cell outline with mScarletI

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpUBE2:mScarletI-Lti6b_3tNos-35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pAJT194

    Plasmid

    #202293

    Purpose

    Type IIS cloning plasmid for LPattB5 integrations w/ OriT

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. hMTP

    Plasmid

    #124281

    Purpose

    Expresses human MTP in mammalian cells.

    Depositor

    Mahmood Hussain

    Article

    Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

    Insert

    MTTP (MTTP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

    Plasmid

    #222993

    Purpose

    Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWM_12x601_193 NRL + TetO

    Plasmid

    #157812

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_tetO
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 3TERM_Nos-35S

    Plasmid

    #136116

    Purpose

    3TERM after CDS/ CDS12-CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    3TERM_Nos-35S (A. tumefaciens, CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA3-SypHluorin 2x (S2x)

    Plasmid

    #37004

    Depositor

    Yongling Zhu, Stephen Heinemann

    Article

    Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

    Insert

    SypHluorin 2x (Syp Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Two copies of pHluorin inserted at site 184-185

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCherry-C1-p63RhoGEF

    Plasmid

    #67896

    Purpose

    Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-p63RhoGEF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. rASAP

    Plasmid

    #178205

    Purpose

    Ratiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.

    Depositor

    Stefan Heinemann

    Article

    Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

    Insert

    rASAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCk3_spacer

    Plasmid

    #136073

    Purpose

    Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCiVSP(C363S)-SD64TF

    Plasmid

    #80334

    Purpose

    Expresses Ciona VSP (without phosphatase activity) in Xenopus Oocyte

    Depositor

    Yasushi Okamura

    Article

    Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

    Insert

    Ciona intestinalis voltage-sensing phosphatase (C363S) (vsp Ciona intestinalis)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    Changed Cysteine 363 to Serine

    Promoter

    SP6

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTA-Luc SIRT1 promoter (-202)

    Plasmid

    #10971

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

    Insert

    SIRT1 promoter (Sirt1 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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