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pATP403red-hENT1

Plasmid

#166027

Purpose

Expresses human equilibrative nucleoside transporter 1 (hENT1)

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

human equilibrative nucleoside transporter 1 (SLC29A1 Human)

Use

Synthetic Biology

Expression

Yeast

Promoter

pTDH3

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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aav-SYN-tdNfsB(F124W)-mCherry (eNTR)

Plasmid

#113760

Purpose

AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoter

Depositor

Luke Lavis

Article

Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

Insert

tdNfsB(F124W)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

F124W

Promoter

Synapsin

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMIG-N19RhoA

Plasmid

#14120

Depositor

Richard Hynes

Article

Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

Insert

N19 Human RhoA (RHOA Human)

Use

Retroviral

Tags

Myc

Mutation

Site directed mutagenesis of Thr to Asn at Codon …

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
Flag-Craf SSDD

Plasmid

#118337

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf SSDD (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

SSDD

Promoter

CMV

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN

Plasmid

#73365

Purpose

Expresses myc tagged XPLN in mammalian cells

Depositor

Jie Chen, Krister Wennerberg

Article

Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pLEX-FKBP-Rab11-Blasticidin

Plasmid

#120717

Purpose

Expresses recycling endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab11a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Amino acids 2-216

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-ALK6-HA

Plasmid

#11769

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK6 (Bmpr1b Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pETDuet RagA(D130N)-RagC

Plasmid

#99642

Purpose

Bacterial expression of RagA(D130N)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

D130N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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HLCS_pCSdest

Plasmid

#53803

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

HLCS (HLCS Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pETDuet RagA(T21N)-RagC

Plasmid

#99644

Purpose

Bacterial expression of RagA(T21N)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

T21N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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rTetR-GFP-VP16

Plasmid

#183917

Purpose

VP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

rTetR-GFP-VP16 (UL48 Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pISceI Dusp6:memGFP

Plasmid

#32668

Purpose

Expresses membrane localized EGFP under the Dusp6 promoter

Depositor

Michael Tsang

Article

Wang et al Development. 2011 Jan;138(1):45-54. Epub

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Available Since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR_hEMSY

Plasmid

#125156

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

EMSY (EMSY Human)

Use

Gateway entry vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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p5077 pcDNA4c hBrd4 (1-594)

Plasmid

#14453

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1-594) (BRD4 Human)

Tags

His

Expression

Mammalian

Mutation

amino acids 1-594

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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Orai1

Plasmid

#12199

Depositor

Anjana Rao

Article

Feske et al Nature. 2006 May 11. 441(7090):179-85.

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pETDuet RagA-RagC(D181N)

Plasmid

#99643

Purpose

Bacterial expression of RagA-RagC(D181N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

D181N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Ift52

Plasmid

#128875

Purpose

For mammalian expression of mouse Ift52

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Ift52 (Ift52 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3/hArf3(WT)-EGFP

Plasmid

#79418

Purpose

Expresses C-terminally EGFP-tagged Arf3(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (ARF3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_human-4xUAS

Plasmid

#125150

Purpose

Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Stap-seq screening vector

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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NG0886 pCI-TPI-WT-4H

Plasmid

#65801

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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