We narrowed to 7,280 results for: 11

1. pMCh-664

   Plasmid

   #92252

   Purpose

   Plasmid for expression of GST-tagged human TNRC6C CED with mutated PAM2 motif in mammalian cells

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   TNRC6C CED (TNRC6C Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   C-terminal effector domain (CED): aa 1369-1690 wi…

   Promoter

   EF1A

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCh-658

   Plasmid

   #92257

   Purpose

   Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motif

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   TNRC6C CED (TNRC6C Human)
   

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   C-terminal effector domain (CED): aa 1369-1690 wi…

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSTARR-seq_SCP1 vector_blocking 2

   Plasmid

   #99317

   Purpose

   Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Hstarr-seq screening vector

   Expression

   Mammalian

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PatoK-OG241

   Plasmid

   #72840

   Purpose

   NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoK
   

   Expression

   Bacterial

   Promoter

   PatoK

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PatoL-LOG241

   Plasmid

   #72846

   Purpose

   Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoL
   

   Expression

   Bacterial

   Promoter

   PatoL

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PatoK-LOG241

   Plasmid

   #72848

   Purpose

   NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoK
   

   Expression

   Bacterial

   Promoter

   PatoK

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pClneoEGFPC-let60 K16N

   Plasmid

   #66861

   Purpose

   Express GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

   Insert

   Let-60 (let-60 Nematode)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lysine 16 is mutated to asparagine

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A

   Plasmid

   #65660

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed glutamic acid 97 to alanine, lysine 100 t…

   Promoter

   CMV, 2x Tet

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

   Plasmid

   #52898

   Purpose

   YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Ypd*1-L-IFP-F[2]
   

   Tags

   L-IFP-F[2]

   Expression

   Yeast

   Mutation

   Changed Tryptophane 80 to Alanine

   Promoter

   GAL1

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

10. TALEN-CCR5L18-NtermN3

    Plasmid

    #51448

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with N3 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) N3 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K150Q, K153Q, and R154Q

    Promoter

    CMV

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. TALEN-CCR5R18-NtermN3

    Plasmid

    #51449

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N3 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N3 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q, K153Q, and R154Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEBG MKK3b (Glu)

    Plasmid

    #50451

    Purpose

    activates p38 in the signaling pathway

    Depositor

    Roger Davis

    Article

    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

    Insert

    MKK3b (MAP2K3 Human)
    

    Tags

    Gst

    Expression

    Mammalian

    Mutation

    changed Serine 218 to Glutamic Acid, changed Thre…

    Promoter

    SV40

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

13. 1287 p3E-mut let-7 etv2 3’ UTR

    Plasmid

    #49011

    Purpose

    Zebrafish Long length etv2 3' UTR with five let7 binding site mutations in gateway 3' Entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    ets variant gene 2 (etsrp Zebrafish)
    

    Use

    Gateway 3' entry

    Mutation

    Mutated the Let7 miRNA binding sites

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pFM038

    Plasmid

    #40141

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    R183-5A + 7SA; base pairs 6-523 of par-2 were re…

    Promoter

    pie-1

    Available Since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pFM101

    Plasmid

    #40147

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    gfp::par-2 wild-type RNAi resistant (par-2 Nematode)
    

    Tags

    GST and eGFP

    Expression

    Bacterial

    Mutation

    Base pairs 6-523 of par-2 were recoded to create …

    Promoter

    tac

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pFM102

    Plasmid

    #40148

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    gfp::par-2 RNAi resistant R183-5A (par-2 Nematode)
    

    Tags

    GST and eGFP

    Expression

    Bacterial

    Mutation

    R183-5A; base pairs 6-523 of par-2 were recoded t…

    Promoter

    tac

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pFM041

    Plasmid

    #40144

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (S241A) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    S241A; base pairs 6-523 of par-2 were recoded to…

    Promoter

    pie-1

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pFM039

    Plasmid

    #40142

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    R183-5A + S241A; base pairs 6-523 of par-2 were …

    Promoter

    pie-1

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pFM036

    Plasmid

    #40139

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (K162A) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    K162A; base pairs 6-523 of par-2 were recoded to…

    Promoter

    pie-1

    Available Since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pFM052

    Plasmid

    #40146

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant (C56S) (par-2 Nematode)
    

    Use

    Gateway destination vector

    Tags

    GFP

    Expression

    Worm

    Mutation

    C56S; base pairs 6-523 of par-2 were recoded to …

    Promoter

    pie-1

    Available Since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only