We narrowed to 5,494 results for: SPI

1. pCAG-tdTomato

   Plasmid

   #83029

   Purpose

   expresses tdTomato fluorescent protein under the CAG promoter

   Depositor

   Angelique Bordey

   Article

   Pathania et al PLoS One. 2012;7(5):e38174. doi: 10.1371

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGI3EM17

   Plasmid

   #210569

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
   

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpH2Bpro

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEK39

   Plasmid

   #210599

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
   

   Use

   Tags

   mRuby3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpH2Bpro

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEM112B

   Plasmid

   #210602

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
   

   Use

   Tags

   mClover3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpH2A/Bpro (divergent)

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLaNGo11-7

   Plasmid

   #126055

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   chloroplastic FNR transit peptide
   ccdB cassette (BsaI)
   GFP11x7 (5 aa linker)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-mGFP-Actin

   Plasmid

   #21948

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

   Insert

   betaActin (ACTB Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRH029

   Plasmid

   #171060

   Purpose

   Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   Gag-spyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x SV40 NLS and 3x FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-SEP-GluR1

   Plasmid

   #24000

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   GluR1 (Gria1 Rat)
   

   Use

   Tags

   GluR1 signal peptide (aa1-22) and SEP (supereclip…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY016 (pcDNA3.1-hLbCpf1)

   Plasmid

   #69988

   Purpose

   Expresses humanized LbCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. LbCas12a-niCPE

    Plasmid

    #213747

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-nLbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, R1138A in LbCas12a

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEGFP-HRas

    Plasmid

    #18662

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-SEP-NRI

    Plasmid

    #23999

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Tags

    NR1 signal sequence (aa1-20) and SEP (supereclipt…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-LacR-TopBP1

    Plasmid

    #31317

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

    Insert

    TopBP1 (TOPBP1 Human)
    

    Use

    Tags

    FLAG and LacR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-Myc-14-3-3ζ

    Plasmid

    #48798

    Purpose

    expresses human myc-14-3-3ζ in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Insert

    YWHAZ (YWHAZ Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG_hPPP1R15B_4-713_mCherry_UK1298

    Plasmid

    #80707

    Purpose

    mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)

    Depositor

    David Ron

    Article

    Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife

    Insert

    human PPP1R15B (PPP1R15B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    CDS 4-713 no stop codon

    Promoter

    CMV

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ230 (LbCpf1)

    Plasmid

    #86210

    Purpose

    LbCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAT009

    Plasmid

    #171635

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. HypoxCR

    Plasmid

    #59946

    Purpose

    A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

    Depositor

    Anne Le

    Article

    Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

    Inserts

    GFP-Pest
    mCherry-Geminin
    

    Use

    Lentiviral

    Tags

    FLAG and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and min CMV

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-MLS-KillerRed

    Plasmid

    #115517

    Purpose

    Middle entry plasmid containing membrane bound KillerRed fluorophore

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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