164,577 results
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5` CC: Puro – loxP – GFP-C
Plasmid#219563Purpose5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.DepositorInserthPGK-promoter_PuroR_2A_loxP_GFP-C
UseUnspecifiedTagsExpressionMutationPromoterhPGKAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTKRED
Plasmid#41062DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-Cas9-His
Plasmid#47327PurposeFor in vitro expression and purification of Cas9 proteinDepositorInsertCas9
UseCRISPRTagsHisExpressionBacterialMutationPromoterAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
secreted BirA-Flag
Plasmid#64395PurposeFlag-tagged biotinylation enzyme for co-expression with bio-proteinsDepositorAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
evoFERNY-Cas9NG
Plasmid#203329PurposePlasmid for bacterial purification of codon optimized evoFERNY-Cas9NGDepositorInsertevoFERNY-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
phCMV-GALV-MTR
Plasmid#163612PurposeRetro and Lentiviral transduction of primary human germinal center B cellsDepositorInsertGaLV MTR envelope
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMG-AIS
Plasmid#225133PurposeFor genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-PE7-SB-NLS
Plasmid#228870PurposePE7 connected to MLH1-SB-NLS via 2A sequenceDepositorInsertPE7-2A-MLH1-SB-NLS
UseCRISPRTagsExpressionMutationPromoterAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ALK
Plasmid#23917DepositorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
LEGO-AP1x6-GM55
Plasmid#217415PurposeLuciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x6DepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoter6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…Available SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUD-MSP1D1
Plasmid#161608PurposeBacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1; all tryptophans and tyrosines w…PromoterT7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-mCasp8
Plasmid#208782PurposeInducibly expresses mouse caspase8 fused to light-activatable Cry2DepositorInsertmCherry mCaspase8 Cry2 (Casp8 Mouse)
UseLentiviralTagsCry2 and mCherryExpressionMammalianMutationPromoterAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1_Puro
Plasmid#125594PurposeLentiviral expression of sgRNA with GFP and puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD33-mf-lon
Plasmid#21867DepositorInsertLon (Mfl404 Mesoplasma florum)
UseTagsExpressionBacterialMutationall TGA codons changed to TGG codonsPromoterAvailable SinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
dCAS9-VP64_GFP
Plasmid#61422PurposeExpresses dCAS9-VP64 activator with 2A GFPDepositorHas ServiceConcentrated Lentiviral PrepInsertdCAS9(D10A,H840A)-VP64_2A_GFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and H840A in Cas9PromoterEF1AAvailable SinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKNOCK-Cm
Plasmid#46261PurposeBacterial targetingDepositorTypeEmpty backboneUseBacterial targetingTagsExpressionMutationPromoterAvailable SinceAug. 14, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKLV2-EF1a-BsdCas9-W
Plasmid#67978PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…ExpressionMutationPromoterEF1aAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopCatcher
Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
UseTagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGH125_dCas9-Blast
Plasmid#85417Purposelentiviral expression vector for dCas9 with Blasticidin selectable markerDepositorInsertdCas9 and Blasticidin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only
