We narrowed to 5,300 results for: pcDNA3.1+

1. Vh-Clover-CC-M2-mRuby2-Vt

   Plasmid

   #233231

   Purpose

   Vinculin FRET tension sensor with coiled-coil M2 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-M2-mRuby2-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Vinculin-Clover-CC-Mad-mRuby2

   Plasmid

   #233239

   Purpose

   Vinculin FRET tension sensor with coiled-coil Mad domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-Mad-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Vh-Clover-CC-S1-mRuby2-Vt

   Plasmid

   #233230

   Purpose

   Vinculin FRET tension sensor with coiled-coil S1 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S1-mRuby2-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Vh-Clover-CC-S0-mRuby2-Vt

   Plasmid

   #233226

   Purpose

   Vinculin FRET tension sensor with coiled-coil S0 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S0-mRuby2-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Vh-Clover-CC-S4-mRuby2-Vt

   Plasmid

   #233228

   Purpose

   Vinculin FRET tension sensor with coiled-coil S4 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S4-mRuby2-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Vh-Clover-CC-S7-mRuby2-Vt

   Plasmid

   #233229

   Purpose

   Vinculin FRET tension sensor with coiled-coil S7 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S7-mRuby2-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Vh-Clover-CC-Mad-mRuby2-Vt

   Plasmid

   #233232

   Purpose

   Vinculin FRET tension sensor with coiled-coil Mad domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-Mad-mRuby2-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Vinculin-Clover-CC-S0-mRuby2

   Plasmid

   #233233

   Purpose

   Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S0-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-Sga-Cas9

   Plasmid

   #227005

   Purpose

   Mammalian expression of Nuclease active S. gallolyticus Cas9

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-Sin-Cas9

    Plasmid

    #227006

    Purpose

    Mammalian expression of Nuclease active S. iniae Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-Spa-Cas9

    Plasmid

    #227007

    Purpose

    Mammalian expression of Nuclease active S. parasanguinis Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-Sub-Cas9

    Plasmid

    #227008

    Purpose

    Mammalian expression of Nuclease active S. uberis Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mproSAAS (21 kDa)

    Plasmid

    #231634

    Purpose

    Mammalian expression of the N-terminal domain of the mouse chaperone protein proSAAS-(1-180)

    Depositor

    Iris Lindberg

    Article

    Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

    Insert

    Pcsk1N (Pcsk1n Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of C-terminus (aa181-225)

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SARS-CoV spike all5KO mutant

    Plasmid

    #231921

    Purpose

    For expression of SARS-CoV spike protein with glycan deletion at N1080, N1116, N1140, N1155, N1176 by implementing Asn-to-Gln mutation

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

    Insert

    spike all5KO (S SARS-CoV)
    

    Use

    Tags

    C9 and CD5 signal peptide

    Expression

    Mammalian

    Mutation

    N1080Q, N1116Q, N1140Q, N1155Q, N1176Q

    Promoter

    CMV

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTBL435

    Plasmid

    #226628

    Purpose

    pcDNA-dTdT

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Insert

    catalytically deactivated TdT (DNTT Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    D343E, D345E

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cas9-FCPF

    Plasmid

    #220141

    Purpose

    Expresses Cas9-FCPF in mammalian cells

    Depositor

    Xinlai Cheng

    Article

    Gama-Brambila et al JACS Au. 2021 May 12;1(6):777-785. doi:

    Insert

    Cas9-FCPF (cas9 Synthetic, S. Pyogenes)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA

    Plasmid

    #201964

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    E162A,M164A,R167A

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA

    Plasmid

    #201965

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    E198A, R199A,E200A, D201A

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA

    Plasmid

    #201967

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    R232A, K233A, R234A, K235A, R236A

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA

    Plasmid

    #201968

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    W255A,N262E,K266E

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge