Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,964 results for: BASE

Clear keywords
Showing: 9601 - 9650 of 12964 results

  2. pWT065c

    Plasmid
    #107913
    Purpose
    W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells
    Depositor
    David Liu
    Article
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Insert
    (TCF-LEF)7-BE3-P2A-Luc
    Expression
    Mammalian
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107913-plasmid-map-sequence-id-274317

    Enlarge

  5. pEF1-puro/hpb1

    Plasmid
    #59976
    Purpose
    integrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expression
    Depositor
    Timothy Springer
    Insert
    human integrin b1 headpiece (ITGB1 Human)
    Tags
    Base coiled-coil and His-tag
    Expression
    Mammalian
    Promoter
    EF1a promoter
    Available Since
    Sept. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pEF1-puro/hpb1(short)

    Plasmid
    #59973
    Purpose
    integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression
    Depositor
    Timothy Springer
    Insert
    human integrin b1 (short) (ITGB1 Human)
    Tags
    Base coiled-coil and His-tag
    Expression
    Mammalian
    Promoter
    EF1a promoter
    Available Since
    Sept. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. p3360_KanR_2p

    Plasmid
    #247302
    Purpose
    Production of pinocembrin in Escherichia coli, cloned by BASIC assembly, based on 3360 in Carbonell et al. 2018
    Depositor
    Alexandre Kuhn
    Inserts
    AtCHI
    AtCHS
    Sc4CL
    AtPAL
    Expression
    Bacterial
    Promoter
    Ptrc with lac operon
    Available Since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    247302-plasmid-map-sequence-id-492980

    Enlarge

  14. p3386_KanR_7p

    Plasmid
    #247309
    Purpose
    Production of pinocembrin in Escherichia coli, cloned by BASIC assembly, based on 3386 in Carbonell et al. 2018
    Depositor
    Alexandre Kuhn
    Inserts
    AtCHI
    Sc4CL
    AtCHS
    AtPAL
    Expression
    Bacterial
    Promoter
    PlacUV5 with lac operon
    Available Since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    247309-plasmid-map-sequence-id-492982

    Enlarge

  15. p3387_KanR_4p

    Plasmid
    #247310
    Purpose
    Production of pinocembrin in Escherichia coli, cloned by BASIC assembly, based on 3387 in Carbonell et al. 2018
    Depositor
    Alexandre Kuhn
    Inserts
    AtCHI
    AtCHS
    Sc4CL
    AtPAL
    Expression
    Bacterial
    Promoter
    PLacUV5
    Available Since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    247310-plasmid-map-sequence-id-492981

    Enlarge

  16. pB06_3360_6p

    Plasmid
    #247311
    Purpose
    Production of pinocembrin in Escherichia coli, cloned by BASIC assembly, based on 3360 in Carbonell et al. 2018
    Depositor
    Alexandre Kuhn
    Inserts
    AtCHI
    AtCHS
    Sc4CL
    AtPAL
    Expression
    Bacterial
    Promoter
    Ptrc with lac operator and none
    Available Since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    247311-plasmid-map-sequence-id-492979

    Enlarge

  17. p3382_KanR_7p

    Plasmid
    #247308
    Purpose
    Production of Pinocembrin in Escherichia coli, cloned by BASIC assembly, based on 3382 in Carbonell et al. 2018
    Depositor
    Alexandre Kuhn
    Inserts
    AtCHI
    Sc4CL
    AtCHS
    AtPAL
    Expression
    Bacterial
    Promoter
    PLacUV5 with lac operator and Ptrc with lac opera…
    Available Since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    247308-plasmid-map-sequence-id-492976

    Enlarge

  18. pAAV-VTKS4-GCaMP6f

    Plasmid
    #209931
    Purpose
    Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter. Based on construct from Pouchelon et al Cell Rep Methods. 2022.
    Depositor
    Jessica Cardin
    Insert
    GCaMP6f
    Use
    AAV, CRISPR, and Cre/Lox
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    209931-plasmid-map-sequence-id-414074

    Enlarge

  21. pVP076

    Plasmid
    #222028
    Purpose
    Empty spectinomycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Type
    Empty backbone
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Mutation
    All Esp3i sites domesticated
    Available Since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222028-plasmid-map-sequence-id-447309

    Enlarge

  23. pSBbi-Pur-Lyn-AktAR2-EV-D

    Plasmid
    #125200
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV-D
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125200-plasmid-map-sequence-id-243591

    Enlarge

  27. SARS-CoV-2 S HexaPro

    Plasmid
    #154754
    Purpose
    Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant
    Depositor
    Jason McLellan
    Article
    Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505
    Insert
    Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Available Since
    July 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154754-plasmid-map-sequence-id-304640

    Enlarge

  28. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Article
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

    Enlarge

  29. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid
    #179905
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…
    Promoter
    CAG
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    179905-plasmid-map-sequence-id-351283

    Enlarge

  30. GCN5 core-dCas9-p300 core

    Plasmid
    #179553
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179553-plasmid-map-sequence-id-354783

    Enlarge

  31. pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK

    Plasmid
    #177868
    Purpose
    Cloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d
    Insert
    mGreenLantern-based EXSISERS
    Use
    CRISPR, Synthetic Biology, and Unspecified
    Tags
    OLLAS
    Expression
    Mammalian
    Available Since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    177868-plasmid-map-sequence-id-358838

    Enlarge

  32. p300 core-dCas9-CBP core

    Plasmid
    #179559
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) & c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179559-plasmid-map-sequence-id-354771

    Enlarge

  35. pTRIP TRE BI_SEPT9_i1-14-GFP11_SEPT9_i1-14-GFP10

    Plasmid
    #180351
    Purpose
    mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT9_i1 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN9_v1 (SEPTIN9 Human)
    SEPTIN9_v1 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Promoter
    bidirectional TRE
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180351-plasmid-map-sequence-id-357912

    Enlarge

  36. GCN5 core-dCas9-CBP core

    Plasmid
    #179555
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179555-plasmid-map-sequence-id-354784

    Enlarge

  46. pTRIP TRE BI_SEPT9_i3-14-GFP11_SEPT9_i3-14-GFP10

    Plasmid
    #180352
    Purpose
    mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT9_i3 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN9_v3 (SEPTIN9 Human)
    SEPTIN9_v3 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Promoter
    bidirectional TRE
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180352-plasmid-map-sequence-id-357936

    Enlarge

  47. pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2

    Plasmid
    #180353
    Purpose
    mammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN7 (SEPTIN7 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 H279D
    Promoter
    bidirectional TRE
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180353-plasmid-map-sequence-id-357939

    Enlarge

  48. pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2

    Plasmid
    #180359
    Purpose
    mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 H279D
    Promoter
    bidirectional TRE
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180359-plasmid-map-sequence-id-358019

    Enlarge

  49. pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2

    Plasmid
    #180361
    Purpose
    mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 H279D
    Promoter
    bidirectional TRE
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180361-plasmid-map-sequence-id-358006

    Enlarge

Showing: 9601 - 9650 of 12964 results