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Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-FLEx-axon-GCaMP6s

Plasmid

#112010

Purpose

For cre-dependent enriched expression of GCaMP6s in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV5 and AAV9

Insert

axon-GCaMP6s

Use

AAV and Cre/Lox

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Rab4a-C-7

Plasmid

#231781

Purpose

Visualization of endosome in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Rab4a-C-7 (RAB4A Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-15F11-HA-mEGFP

Plasmid

#129590

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCDH-hMALAT1

Plasmid

#118580

Purpose

to overexpress human MALAT1 in mammalian cells

Depositor

Jianjun Zhao

Article

Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

Insert

MALAT1 (MALAT1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_hsTRPA1_myc_dTom

Plasmid

#183179

Purpose

myc tagged hsTRPA1 used for cell transfection

Depositor

Sreekanth Chalasani

Article

Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

Inserts

hsTRPA1 (TRPA1 Human)
dTom

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-15F11-HA-Halo

Plasmid

#129592

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV_OHu19986C_myc tag

Plasmid

#183174

Purpose

used to make AAV vectors for hsTRPA1 tagged with myc

Depositor

Sreekanth Chalasani

Article

Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

Insert

hsTRPA1 (TRPA1 Human)

Use

AAV

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124428

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-kdrl:mCherry-caax

Plasmid

#194287

Purpose

Expression plasmid for zebrafish or Danionella cerebrum

Depositor

Pui-Ying Lam

Article

Lam et al Dis Model Mech. 2022 Dec 1;15(12):dmm049

Insert

kdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)

Use

Zebrafish or danionella cerebrum expression

Tags

mCherryCAAX from the Tol2kit (Kwan et al., 2007)

Promoter

kdrl(flk1)

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3)P Probe

Plasmid

#211508

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

FYVE-Hrs x 2 (PIKFYVE Human)

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pET23b-15F11-HA-mEGFP-6xHis

Plasmid

#129593

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

Plasmid

#132411

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRfxCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R239A; H244A; R858A; H863A

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

Plasmid

#132407

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dLwaCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R474A; H479A; R1046A; R1051A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Ataxin3Q28

Plasmid

#22122

Depositor

Henry Paulson

Article

Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

Insert

Ataxin 3 (ATXN3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Ataxin3Q84

Plasmid

#22123

Depositor

Henry Paulson

Article

Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

Insert

Ataxin 3 (ATXN3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4/hL3MBTL2

Plasmid

#28232

Depositor

Danny Reinberg

Article

Trojer et al Cell. 2007 Jun 1. 129(5):915-28.

Insert

l(3)mbt-like 2 (Drosophila) (L3MBTL2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only
pK18msB

Plasmid

#177839

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

Depositor

Christopher Johnson

Article

Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

Type

Empty backbone

Use

Bacterial gene replacement

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pmax pona 12xTRICK 24xMS2SL

Plasmid

#64542

Purpose

TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Tags

24xMS2 stem loops, HA, NLS, and dNP799

Expression

Mammalian

Promoter

ponA

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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