We narrowed to 5,107 results for: PEP

1. pET28a(+) 6xHis-TEV-IFIT5

   Plasmid

   #53560

   Purpose

   bacterial expression of IFIT5

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT5 (IFIT5 Human)
   

   Use

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pJ414-PCY1

   Plasmid

   #98150

   Purpose

   Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.4_7C11-1 light chain

   Plasmid

   #198252

   Purpose

   recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)
   

   Use

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.4_7C11-1 heavy chain

   Plasmid

   #198251

   Purpose

   recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)
   

   Use

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

   Plasmid

   #170183

   Purpose

   Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   sgBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC93

   Plasmid

   #118911

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
   

   Use

   Tags

   eGFP-HIS6

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-HsSENP5

   Plasmid

   #71468

   Purpose

   Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 5 (SENP5 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pSCM167.2-Venus-VF-hADAM10(aa455-550)

   Plasmid

   #189556

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains only disintegrin domain--amino acids 455-550

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   contains aa 455-550

   Promoter

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSCM167-Venus-VF-hADAM10(aa220-459)

   Plasmid

   #189555

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains only metalloprotease domain--amino acids220-459

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   contains aa 220-459

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSCM167.2-Venus-VF-hADAM10(aa551-654)

    Plasmid

    #189557

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only cysteine rich domain--amino acids 551-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa551-654

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

    Plasmid

    #189562

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    contains aa 1-213

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSCM167-Venus-VF-hADAM10(aa455-654)

    Plasmid

    #189561

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only disintegrin and cysteine rich domains--amino acids 455-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 455-654

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GST-SmB'

    Plasmid

    #1842

    Depositor

    Philip Leder

    Article

    Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

    Insert

    SmB' (SNRPB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pEGFP ratAPOBEC1 L173A/G227A

    Plasmid

    #167169

    Purpose

    Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 173 to Alanine; changed Glycine 2…

    Promoter

    CMV promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOCC121

    Plasmid

    #118893

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and MBP, cleavab…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCIneo-HA-Ago2

    Plasmid

    #115362

    Purpose

    Expression vector to produce HA-tagged human Ago2 protein without N-peptide

    Depositor

    Witold Filipowicz, Ramesh Pillai

    Article

    Pillai et al RNA. 2004 Oct . 10(10):1518-25.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQTEV-GTF2F2

    Plasmid

    #31323

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    general transcription factor IIF (GTF2F2 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCellFree_G03 NFKB1

    Plasmid

    #67126

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    NFKB1 (NFKB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pOCC70

    Plasmid

    #118878

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    mGFP, cleavable with TEV protease; HIS6, cleavabl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc-­His-­zfTpo

    Plasmid

    #64309

    Purpose

    Express soluble His-tagged zebrafish thrombopoietin in insect cells

    Depositor

    Petr Bartunek

    Article

    Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

    Insert

    Thrombopoietin (thpo Zebrafish)
    

    Use

    Tags

    (His)6

    Expression

    Insect

    Mutation

    Thrombopoietin starts at AA37 (AA1-36 encode the …

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only