We narrowed to 4,886 results for: phy

1. pEGB 35S:dCas9:Tnos (GB1191)

   Plasmid

   #68223

   Purpose

   Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   35S

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-CARMA2cl-FLAG

   Plasmid

   #97077

   Purpose

   Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

   Depositor

   Pasquale Vito

   Article

   Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

   Insert

   CARD14 isoform 2 (CARD14 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

   Plasmid

   #184381

   Purpose

   This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Cas9, two gRNAs targeting dystrophin
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. integrin beta6 777T pcDNA1 neo

   Plasmid

   #13582

   Depositor

   Dean Sheppard

   Article

   Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

   Insert

   integrin beta 6 777T (ITGB6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   777T. Lacks last 11 amino acids of cytoplasmic do…

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDN-T1TCbt

   Plasmid

   #44514

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
   tetR::ymcherry
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Three tandem tet operators (3XtetO2) downstream o…

   Promoter

   PGal1-T123 promoter

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDN-T1GZmbh

   Plasmid

   #44522

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
   yEGFP::ZeoR
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Three tandem tet operators (3XtetO2) downstream o…

   Promoter

   PGal1-T123 promoter

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDN-G1TCbt

   Plasmid

   #44515

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
   tetR::ymcherry
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Two tandem tet operators (2XtetO2) downstream of …

   Promoter

   PGal1-D12 promoter

   Available since

   April 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. ORAI1-YFP T184M

   Plasmid

   #114181

   Purpose

   ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).

   Depositor

   Nicolas Demaurex

   Article

   Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

   Insert

   ORAI1-YFP T184M (ORAI1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   T184M

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRC/RSV-rat Agt

   Plasmid

   #122104

   Purpose

   Expression of angiotensinogen

   Depositor

   John Chan

   Article

   Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

   Insert

   angiotensinogen (Agt Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV promoter

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDN-G1GZmbh

    Plasmid

    #44516

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    yEGFP::ZeoR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL4 Cluster Promoter Delta 1.2

    Plasmid

    #100130

    Purpose

    It contains 0.5 Kb of the miR-183-96-182 cluster promoter. This fragment partially loses promoter activity in melanoma cells.

    Depositor

    Eva Hernando

    Article

    Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

    Insert

    miR-183-96-82 Cluster promoter 0.5 Kb Fragment (MIR182 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL4-Cluster Promoter Delta 1.1

    Plasmid

    #100129

    Purpose

    It contains ~0.8 Kb of the miR-183-96-182 cluster promoter. Is the minimal region that contains the maximal promoter activity

    Depositor

    Eva Hernando

    Article

    Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

    Insert

    miR-183-96-82 Cluster promoter 0.8 Kb Fragment (MIR182 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. HNF4A_P2-285/-192

    Plasmid

    #31053

    Depositor

    Gerhart Ryffel

    Article

    Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    -285 to -1 of P2 HNF4A promoter with mutation -19…

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. integrin beta6 777T pBabe puro

    Plasmid

    #13583

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-CARMA2cl-HA

    Plasmid

    #97195

    Purpose

    Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 2 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HNF4A_P2-285/-169

    Plasmid

    #31052

    Depositor

    Gerhart Ryffel

    Article

    Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    -285 to -1 of P2 HNF4A promoter with mutation -16…

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HNF4A_P2-285/-136

    Plasmid

    #31051

    Depositor

    Gerhart Ryffel

    Article

    Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

    Insert

    HNF4A P2 promoter (HNF4A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    -285 to -1 of P2 HNF4A promoter with mutation -13…

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p-OiRS3GG

    Plasmid

    #98589

    Purpose

    Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

    Depositor

    Lydia Contreras

    Article

    Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

    Inserts

    group I intron
    PheS cassette to be selectively replaced by unique asRNA probes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD and pLtetO

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pD10AH840AhCas9 (GB1041)

    Plasmid

    #68207

    Purpose

    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge