162,408 results

1. pUCmini-iCAP-AAV9-X1.1

   Plasmid

   #196836

   Purpose

   non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

   Insert

   Synthetic construct isolate AAV9-X1.1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_ENVLPE+

   Plasmid

   #232427

   Purpose

   Expression plasmid to produce ENVLPE+ particles

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   ENVLPE+
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   2xCMV

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS598 EF1alpha-EGFPcre

   Plasmid

   #11923

   Purpose

   Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

   Depositor

   Brian Sauer

   Article

   Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

   Insert

   EGFP-cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-TurboID

   Plasmid

   #124646

   Purpose

   Expresses Flag-TurboID fusion for proximity labeling

   Depositor

   Ken-Ichi Takemaru, FENG-QIAN Li

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-PPARA

   Plasmid

   #169019

   Purpose

   Expression of PPARA driven by CMV promoter

   Depositor

   Claes Wadelius

   Article

   Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

   Insert

   human ppara (PPARA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBBR15.2-2H12.D11opt-scarRef

   Plasmid

   #221150

   Purpose

   cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen2.1
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen2.1)

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJR98

   Plasmid

   #187239

   Purpose

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUAS-NanoLuc

   Plasmid

   #87696

   Purpose

   Gal4VP16 driven expression of NanoLuc

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   NanoLuc
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW-Cas9-Blast

    Plasmid

    #83481

    Purpose

    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

    Depositor

    Mohan Babu

    Inserts

    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    Tight Tre and human phosphoglycerate kinase 1 pro…

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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