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We narrowed to 8,888 results for: tre promoter

Showing: 7721 - 7760 of 8888 results

  1. pSCBE3-NG-Hypa

    Plasmid
    #218161
    Purpose
    This plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N692A…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218161-plasmid-map-sequence-id-433271

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  2. peSCBE3-NG-Hypa

    Plasmid
    #218165
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218165-plasmid-map-sequence-id-433201

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  3. peSCBE3-NG-HF1

    Plasmid
    #218164
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218164-plasmid-map-sequence-id-433171

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  4. N229A (EcQ) MetNIQ in pBAD

    Plasmid
    #118257
    Purpose
    N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (N229A) (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala) and…
    Promoter
    None and araBAD Promoter
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118257-plasmid-map-sequence-id-237124

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  5. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

    Plasmid
    #118260
    Purpose
    N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (Y160A) (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118260-plasmid-map-sequence-id-228496

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  6. pSCBE3-NG-HF1-Hypa

    Plasmid
    #218162
    Purpose
    This plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218162-plasmid-map-sequence-id-433200

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  7. pETDuet-TFIIE

    Plasmid
    #171083
    Purpose
    Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    TF2E1 (GTF2E1 Human)
    TF2E2 (GTF2E2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171083-plasmid-map-sequence-id-335640

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  9. pCB268

    Plasmid
    #53363
    Purpose
    pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.
    Depositor
    Alexander Varshavsky
    Article
    Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:
    Inserts
    DHFR (Dhfr Mouse)
    Ub
    Aβ42 (APP Human)
    Use
    Tags
    13xMyc, Flag, and HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pGEMHE-RnCENP-O-203-GFP

    Plasmid
    #205183
    Purpose
    Expresses chimeric rat CENP-O with mouse CENP-O middle region including central RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Michael Lampson
    Article
    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208
    Insert
    CENP-O (Cenpo, Cenpo Rat, Mouse)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Chimeric rat CENP-O with mouse CENP-O middle regi…
    Promoter
    T7
    Available since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    205183-plasmid-map-sequence-id-409738

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  12. pGEMHE-RnCENP-O-298-GFP

    Plasmid
    #205184
    Purpose
    Expresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Michael Lampson
    Article
    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208
    Insert
    CENP-O (Cenpo, Cenpo Rat, Mouse)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Chimeric rat CENP-O with mouse CENP-O C-terminal …
    Promoter
    T7
    Available since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    205184-plasmid-map-sequence-id-409741

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  13. pGEMHE-MmCENP-O-R298-GFP

    Plasmid
    #205185
    Purpose
    Expresses chimeric mouse CENP-O with rat CENP-O C-terminal RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Michael Lampson
    Article
    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208
    Insert
    CENP-O (Cenpo, Cenpo Rat, Mouse)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Chimeric mouse CENP-O with rat CENP-O C-terminal …
    Promoter
    T7
    Available since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    205185-plasmid-map-sequence-id-409763

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  14. pGEMHE-RnCENP-O-11NS-GFP

    Plasmid
    #205181
    Purpose
    Expresses rat CENP-O with 11 mouse-specific point mutations in residues under positive selection; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Michael Lampson
    Article
    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208
    Insert
    CENP-O (Cenpo, Cenpo Rat, Mouse)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Rat CENP-O with 11 mouse-specific point mutations…
    Promoter
    T7
    Available since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    205181-plasmid-map-sequence-id-409746

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  18. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Article
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Use
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169168-plasmid-map-sequence-id-332048

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  24. pGS-M1-BRG1-3xFLAG-BAF57-BAF60A

    Plasmid
    #109190
    Purpose
    Plasmid for expression of C-terminally 3xFLAG-tagged BRG1, untagged BAF57 and untagged BAF60A under the control of a p10 promoter in baculovirus/insect cell systems
    Depositor
    Monika Golas
    Article
    Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-
    Inserts
    BRG1 (SMARCA4 Human)
    BAF57 (SMARCE1 Human)
    BAF60A (SMARCD1 Human)
    Use
    Tags
    3xFLAG
    Expression
    Insect
    Mutation
    Promoter
    p10
    Available since
    July 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    109190-plasmid-map-sequence-id-233083

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  33. KG#205

    Plasmid
    #110881
    Purpose
    Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1
    Inserts
    rab-3 promoter
    pde-4d (isoform d) D448N cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Asp 448 to Asn
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110881-plasmid-map-sequence-id-212568

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Showing: 7721 - 7760 of 8888 results