We narrowed to 9,193 results for: Pol;

1. pGEX4T3-GST-NQO1

   Plasmid

   #177140

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of NQO1 with a TEV protease site located between the GST tag and NQO1

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   NQO1 (NQO1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDFDuet-nsp7-nsp8

   Plasmid

   #159092

   Purpose

   Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

   Depositor

   Seth Darst

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Inserts

   NSP7
   NSP8
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30a-His6-LOXCATmut

   Plasmid

   #140085

   Purpose

   A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutation in H265A and R268A in LOX (used original…

   Promoter

   T7

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF myc Gal cyclin T1 (1-726) (P#1026)

   Plasmid

   #14626

   Depositor

   Matija Peterlin

   Article

   Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

   Insert

   cyclin T1 (CCNT1 Human)
   

   Tags

   Gal and myc

   Expression

   Mammalian

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1P CISD1_1

   Plasmid

   #160773

   Purpose

   Suppress CISD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD1_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MCAM-GFP

   Plasmid

   #214475

   Purpose

   Lentiviral vector for the expression of MCAM-GFP fusion protein in mammalian cells.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   MCAM (MCAM Human)
   

   Use

   Lentiviral; Gateway

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGM117

   Plasmid

   #220168

   Purpose

   Expresses a reporter for an alpha-helical OMM protein

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   CISD1 (CISD1 Human)
   

   Use

   Lentiviral

   Tags

   GFP11

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1P CISD1_2

   Plasmid

   #160774

   Purpose

   Suppress CISD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD1_2
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQHA-USP7 WT puroR

   Plasmid

   #46753

   Purpose

   Retroviral vector that expresses wild type HA-tagged human USP7

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP469 pEF1a-mCherry-EMPTY-gRNA

    Plasmid

    #100958

    Purpose

    mCherry driven by EF1a, empty gRNA scaffold driven by U6

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA empty backbone with RFP
    

    Use

    CRISPR

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS1995

    Plasmid

    #49125

    Purpose

    AAV plasmid with GPR88 (Ple94) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple94-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    GPR88

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pGL4_10xUAS-CMVmin-BC0396-luc2

    Plasmid

    #227115

    Purpose

    Barcoded assay, UAS sensor for split TEV assays; barcode BC0396

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    10x clustered UAS element linked to CMV minimal promoter driving barcode 0396 and a luciferase reporter gene
    

    Expression

    Mammalian

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBactin-Tubg1-mEGFP

    Plasmid

    #196870

    Purpose

    Expression of the gamma-tubulin fused to enhanced (E)-GFP

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Tubg1-mEGFP (Tubg1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEE075

    Plasmid

    #176832

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    714
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAaePUb::Cas9-2A-Neo

    Plasmid

    #176680

    Purpose

    Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP

    Plasmid

    #220797

    Purpose

    shRNA plasmid against human SRGAP2B/C genes (#2) with EGFP expression in neurons

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

    Insert

    EGFP
    

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQHA-USP7 CS puroR

    Plasmid

    #46754

    Purpose

    Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C223S--catalytically inactive

    Promoter

    CMV

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. W118-1_hTGM5-Flag

    Plasmid

    #180410

    Purpose

    lentiviral transduction of human TGM5 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM5 (TGM5 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-MCS-shCHD5 #1

    Plasmid

    #68876

    Purpose

    CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-MCS-shCHD5 #2

    Plasmid

    #68877

    Purpose

    CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

21. W118-1_hTGM6-Flag

    Plasmid

    #180411

    Purpose

    lentiviral transduction of human TGM6 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM6 (TGM6 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. SaCas9v2-Puro

    Plasmid

    #178802

    Purpose

    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA5FRT/TO-Strep/HA-UBE2J1

    Plasmid

    #124669

    Purpose

    Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2J1 (UBE2J1 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL4_CRE-CMVmin-BC0491-luc2

    Plasmid

    #227133

    Purpose

    Barcoded assay, cAMP/Ca2+ sensor; barcode BC0491

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered CRE element linked to CMV minimal promoter driving barcode 0491 and a luciferase reporter gene
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-lox-roxSTOProx-tRFP-pA-lox

    Plasmid

    #196888

    Purpose

    Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    turbo (t)RFP
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGL4_NFAT-RE-CMVmin-BC0538-luc2

    Plasmid

    #227140

    Purpose

    Barcoded assay, Ca2+ sensor; barcode BC05038

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered NFAT element linked to CMV minimal promoter driving barcode 05038 and a luciferase reporter gene
    

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LLP453 pEf1a-NLS-aGCN4-mCherry

    Plasmid

    #100945

    Purpose

    mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    scFvGCN4, sfGFP, GB1, mCherry
    

    Tags

    3xTy1

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRMC2-tat:msfGFP

    Plasmid

    #194915

    Purpose

    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Expression

    Bacterial

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox

    Plasmid

    #196889

    Purpose

    Dre-activated Cre-inactivated expression of lyn-mScarlet reporter.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    lyn-mScarlet
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX462-hPRKAA1-gRNA_B

    Plasmid

    #74375

    Purpose

    gRNA_B to knockout human AMPK alpha 1 using Cas9n

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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