We narrowed to 5,566 results for: SPI

1. LbCas12a-niCPE

   Plasmid

   #213747

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-nLbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A in LbCas12a

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-IRES-GFP-human-Caspase-1

   Plasmid

   #183359

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1 (CASP1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. L2383-FRB-GPA-NLuc

   Plasmid

   #222902

   Purpose

   Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-SEP-NRI

   Plasmid

   #23999

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   NR1 (Grin1 Rat)
   

   Use

   Tags

   NR1 signal sequence (aa1-20) and SEP (supereclipt…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-MLS-KillerRed

   Plasmid

   #115517

   Purpose

   Middle entry plasmid containing membrane bound KillerRed fluorophore

   Depositor

   Marco Morsch

   Article

   Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

   Insert

   MLS-KillerRed
   

   Use

   Gateway cloning

   Tags

   MLS

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUBC-popTag-QtE-mScarlet

   Plasmid

   #240101

   Purpose

   Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.

   Depositor

   Liam Holt

   Article

   Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e

   Insert

   Encapsulin
   

   Use

   Lentiviral

   Tags

   mScarlet and popZ

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UBC promoter

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Brachyury-eGFP

   Plasmid

   #21063

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   Brachyury promoter
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG_hPPP1R15B_4-713_mCherry_UK1298

   Plasmid

   #80707

   Purpose

   mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)

   Depositor

   David Ron

   Article

   Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife

   Insert

   human PPP1R15B (PPP1R15B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   CDS 4-713 no stop codon

   Promoter

   CMV

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-SEP-GluR2(Q)

   Plasmid

   #24002

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   GluR2 (Gria2 Rat)
   

   Use

   Tags

   GluR2 signal peptide (aa1-24) and SEP (supereclip…

   Expression

   Mammalian

   Mutation

   R607Q

   Promoter

   CMV

   Available since

   April 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGI3EM17

    Plasmid

    #210569

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2Bpro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LVDP 2P.2

    Plasmid

    #231904

    Purpose

    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPs

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

    Insert

    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R203M in N protein

    Promoter

    CMV, EF-1a

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLaNGo11-7

    Plasmid

    #126055

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. DR5rev::erRFP

    Plasmid

    #61011

    Purpose

    Auxin signalling reporter (DR5rev) driving RFP (ER-targeted)

    Depositor

    Alexis Maizel

    Article

    Marin et al Plant Cell. 2010 Apr;22(4):1104-17. doi:

    Insert

    DR5rev::erRFP:35S_term
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    DR5rev::erRFP:35Sterm

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI SMN1

    Plasmid

    #72286

    Purpose

    SMN1 exon 7 splicing cassette

    Depositor

    Elliot Androphy

    Article

    Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

    Insert

    SMN1 (SMN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    exons 6-7-8 including introns

    Promoter

    CMV

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pEK39

    Plasmid

    #210599

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
    

    Use

    Tags

    mRuby3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2Bpro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_human-pro-IL18

    Plasmid

    #183390

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEM112B

    Plasmid

    #210602

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
    

    Use

    Tags

    mClover3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2A/Bpro (divergent)

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-SARS-CoV-2-HiBiT-N

    Plasmid

    #215815

    Purpose

    Expresses N protein with HiBiT tag in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 HiBiT Nucleocapsid (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAT005

    Plasmid

    #171632

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PTEF1-yEGFPCLN2PEST-pRS406

    Plasmid

    #64406

    Purpose

    TEF promoter driven GFP expression

    Depositor

    Claudia Vickers

    Article

    Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

    Insert

    TEF1 promoter (TEF1 Budding Yeast)
    

    Use

    Tags

    yEGFPCLN2PEST (destabilized yeast EGFP)

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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