We narrowed to 11,800 results for: emb
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Plasmid#176056PurposeEGFP fused to the N-terminus of PolB & a hygromycin resistance cassetteDepositorAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only
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pGBW-m4046292
Plasmid#149062PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;StrepIIExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046381
Plasmid#149010PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7
Plasmid#214140PurposeInsect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7DepositorInsertsTags2xSTIIExpressionInsectPromoterpolyhedrinAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSUC2::GNSI (NrsR)
Plasmid#121537PurposeThis plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-PTPRD-P459L
Plasmid#25644DepositorInsertProtein tyrosine phosphatase receptor-type delta (PTPRD Human)
UseLentiviralExpressionMammalianMutationtumor-derived mutation created by site-directed m…Available sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2FPP
Plasmid#203758PurposeEncodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHRas c-term (HRAS Human)
TagsmEos3.2ExpressionMammalianAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-Macro-Linker-eGFP
Plasmid#176067PurposeEGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassetteDepositorInsertMacro
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS21: pHelper(PmcCAST)_entry
Plasmid#168154PurposeInducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning.DepositorInsertsPmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
ExpressionBacterialAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4046277
Plasmid#145428PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAL612
Plasmid#119860Purposeprecusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 CysteineDepositorInsertouter membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
TagsnoneExpressionBacterialMutationCysterine 290 to Serine and Glycine 244 to Cystei…PromoterlacAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM5085
Plasmid#85122PurposePkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.DepositorInsertPkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
TagsECFPExpressionBacterialAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
EYFP-hDF-deltaTM-pCMV-9
Plasmid#72586Purposehuman dysferlin no transmembrane domain tagged with YFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdysferlin (DYSF Human)
TagsEYFPExpressionMammalianMutationtransmembrane domain deletedPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DPY-pNCS
Plasmid#17365DepositorPublicationInsertMMLV proviral DNA
Mutationdeletion that removes 5 amino acids (DPPPY) from …Available sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
TP1320
Plasmid#252367PurposeExpression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tagDepositorInsertanti-EMC nanobody E2
TagsALFA-sfGFP-SUMOEu and His10ExpressionBacterialMutationContains engineered nanobody variant with ALFA ta…Available sinceApril 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-WWE-Linker-eGFP
Plasmid#176072PurposeEGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassetteDepositorInsertWWE
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133720
Plasmid#152913PurposeMammalian expression plasmid for SARS-CoV-2 M (membrane)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133594
Plasmid#152881PurposeMammalian expression plasmid for SARS-CoV-2 M (membrane)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;V5ExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
2C-EGFP-t2a-mCD4
Plasmid#216213PurposeExpresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD4 (Cd4 Mouse)
TagsGFP-T2AExpressionMammalianMutationCD4 transmembrane domain onlyPromoterMERVLAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only