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Auxiliary 3

Plasmid

#153351

Purpose

Provides Middle-to-End linker 3 for level 2 cloning

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

Middle-to-End linker 3

Use

Synthetic Biology

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary 1

Plasmid

#153349

Purpose

Provides Middle-to-End linker 1 for level 2 cloning

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

Middle-to-End linker 1

Use

Synthetic Biology

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MDC

Plasmid

#108950

Purpose

heterologous expression of MDC of S. cerevisiae in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate decarboxylase (MDC)

Tags

His6-Tag

Expression

Bacterial

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-PMK

Plasmid

#108949

Purpose

heterologous expression of PMK of E. faecalis in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Phosphomevalonate kinase (PMK)

Tags

His6-Tag

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-IDI2

Plasmid

#108951

Purpose

heterologous expression of IDI2 of T. thermophilus in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Isopentenyldiphosphat isomerase 2 (IDI2)

Tags

His6-Tag

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBS cPtc2 (CT#4)

Plasmid

#13942

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 2001 Nov 1. 239(1):15-29.

Insert

Ptc2 (PTCH2 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
lvl0 TUB9p

Plasmid

#153373

Purpose

Plant promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

TUB9 promoter

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-AACT

Plasmid

#108944

Purpose

heterologous expression of AACT of M. sedula in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Acetoacetyl-CoA synthase (AACT)

Tags

His6-Tag

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGST2-yHse1-VHS

Plasmid

#44415

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Hse1-VHS (HSE1 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pTU-A-000

Plasmid

#124409

Purpose

Slot A, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-B-000

Plasmid

#124410

Purpose

Slot B, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-C-000

Plasmid

#124411

Purpose

Slot C, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C + terminator

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-GyrA

Plasmid

#128738

Purpose

Expresses GB1 fused to GyrA intein at the C-terminal

Depositor

Sharon Rozovsky

Article

Liu et al Chembiochem. 2019 Jul 2. doi: 10.1002/cb

Insert

GB1

Tags

GyrA

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+ Sizzled

Plasmid

#16688

Depositor

Marc Kirschner

Article

Salic et al Development. 1997 Dec . 124(23):4739-48.

Insert

Sizzled (szl Frog)

Use

Xenopus expression

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
Sus1/PRSF

Plasmid

#26961

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Sus1 (SUS1 Budding Yeast)

Expression

Bacterial

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
Sgf11/pCDF

Plasmid

#26960

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

sgf11 (SGF11 Budding Yeast)

Expression

Bacterial

Available Since

Jan. 4, 2011

Availability

Academic Institutions and Nonprofits only
ZE2 Wnt-4

Plasmid

#17043

Depositor

Randall Moon

Article

Ungar et al Mech Dev. 1995 Aug;52(2-3):153-64. doi:

Insert

wnt-4 cDNA (wnt4a Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET21a.MJ0697

Plasmid

#11411

Depositor

Sung-Hou Kim

Article

Wang et al EMBO J. 2000 Feb 1. 19(3):317-23.

Insert

fibrillarin homolog (MJ_0697 M. jannaschii)

Expression

Bacterial

Mutation

M222T

Promoter

T7

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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p061 pCS xTRPS1

Plasmid

#11033

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 (trps1 Frog)

Use

Mammalian, avian, and zebrafish

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pYTK049

Plasmid

#65156

Purpose

Encodes I-SceI recognition site as a Type 234 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

I-SceI recognition site

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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