Addgene

Showing: 3901 - 3920 of 9565 results

Results per page:

Sort results by:

pGEX4T2-Snf1-cat

Plasmid

#52683

Purpose

expression of the Snf1 kinase domain in bacteria

Depositor

Benjamin Turk

Article

Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

Insert

SNF1 (SNF1 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes kinase domain only, aa 41-315

Promoter

tac

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Plasmid

#159801

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pXIP TGBRI K to R

Plasmid

#19150

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

Transforming growth factor, beta receptor I (TGFBR2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

K420R mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pTW080

Plasmid

#115928

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/30 SGP NA (No Aptamer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
enAsABE8e-dimer

Plasmid

#138507

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-M2-TLR2-V660N

Plasmid

#12292

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y and V660N; signal peptide (aa1-23) re…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)

Plasmid

#87202

Purpose

To express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 90-107 are inserted.

Promoter

CMV

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Plasmid

#159864

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-M2-TLR2-P681H

Plasmid

#12290

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

A74V, M82I, H202Y and P681H; signal peptide (aa1-…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
CD23b in pIRES2eGFP

Plasmid

#19136

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

CD23 beta (FCER2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-RIPK3 K55R

Plasmid

#78807

Purpose

To overexpress RIPK3 K55R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K55R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_N_LHX4

Plasmid

#167778

Purpose

Expresses LHX4 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

LHX4 (LHX4 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-RIPK3 S199A

Plasmid

#78806

Purpose

To overexpress RIPK3 S199A in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S199A

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST myc MOV10 K530A

Plasmid

#51719

Purpose

plasmid expresses myc-tagged MOV10 K530A

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 K530A (MOV10 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

K530A

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT2

Plasmid

#98306

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

Insert

PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B

Use

Tags

Expression

Yeast

Mutation

ERG20 N127W

Promoter

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Ago3m

Plasmid

#21531

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago3 (AGO3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

missing aa757-823, the C-termianl 66aa of PIWI do…

Promoter

Available since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only
pET28a-Hook3 aa 1-239

Plasmid

#74609

Purpose

Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239 (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]

Plasmid

#159802

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIC28-BSA4-G-S10

Plasmid

#127828

Purpose

Production of recombinant human ribosomal protein S10

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS10 (RPS10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Ets2DBM

Plasmid

#28129

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Use

Mouse Targeting and Retroviral

Tags

Expression

Mammalian

Mutation

The putative destruction box (RGTL) residues 20–2…

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only