We narrowed to 4,063 results for: biorxiv

1. hnRNPA1_allW_D262V-FLAG-IRES-eGFP

   Plasmid

   #234621

   Purpose

   Mammalian expression of full-length hnRNPA1 D262V with all aromatic amino acids in the C-terminal LCD mutated to W keeping the hexapeptide fibril core (SYNDFG) and the PY-NLS intact.

   Depositor

   Tanja Mittag

   Article

   Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

   Insert

   hnRNPA1 allW D262V (HNRNPA1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Famillial ALS mutation D262V, all aromatic amino …

   Promoter

   CMV

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

   Plasmid

   #229810

   Purpose

   A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-AmCyan-stop-lox
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

   Plasmid

   #229814

   Purpose

   A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-EGFP-stop-lox
   

   Use

   Cre/Lox; Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

   Plasmid

   #229816

   Purpose

   A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-EGFP-stop-lox
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

   Plasmid

   #229826

   Purpose

   Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet-I
   

   Use

   AAV

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

   Plasmid

   #229841

   Purpose

   A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-H2B-mCerulean-2xStop-loxP
   

   Use

   Cre/Lox; Tol2 based expression vector

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. 11_T7-SP-mScarlet3-GPI

   Plasmid

   #229806

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment site

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   SP-mScarlet3-GPI
   

   Use

   In vitro transcription

   Mutation

   T7 promoter

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFB1.HMBP.PrS.CBX8ΔChromo

   Plasmid

   #224708

   Purpose

   Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   CBX8 chromodomain truncation (CBX8 Human)
   

   Tags

   HMBP

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFBOH-MHL.mEGFP-CBX8

   Plasmid

   #224710

   Purpose

   Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   CBX8 (CBX8 Human)
   

   Tags

   mEGFP

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Oct. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pFB1.HMBP.PrS.CBX8-KR21A

    Plasmid

    #224709

    Purpose

    Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 KR21A mt (CBX8 Human)
    

    Tags

    HMBP

    Expression

    Insect

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB1.HMBP.PrS.CBX8

    Plasmid

    #224707

    Purpose

    Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 (CBX8 Human)
    

    Tags

    HMBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)

    Plasmid

    #194234

    Purpose

    Lentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    EF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LMPd Ametrine Got1 shRNA#1

    Plasmid

    #220592

    Purpose

    Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

    Depositor

    Russell Jones

    Article

    Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

    Insert

    Got1 shRNA (Got1 Mouse)
    

    Use

    Retroviral

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LacI-pLacI-pI pm

    Plasmid

    #218098

    Purpose

    Phagemid that expresses LacI, and pI under the Lac promoter

    Depositor

    Kevin Esvelt

    Insert

    pI
    

    Expression

    Bacterial

    Promoter

    Lac promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. anti-NPY/Neuropeptide Y scFv [L115/13] with sortase tag

    Plasmid

    #204426

    Purpose

    Mammalian Expression of anti-Neuropeptide Y scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L115/13.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting NPY/Neuropeptide Y (Homo sapiens)
    

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. anti-Calbindin scFv [L109/57] with sortase tag

    Plasmid

    #204421

    Purpose

    Mammalian Expression of anti-Calbindin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L109/57.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting Calbindin (Homo sapiens)
    

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. anti-GFAP R416WT scFv [N206B/9] with sortase tag

    Plasmid

    #204420

    Purpose

    Mammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting GFAP R416WT (Homo sapiens)
    

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. anti-Parvalbumin scFv [L114/81] with sortase tag

    Plasmid

    #204423

    Purpose

    Mammalian Expression of anti-Parvalbumin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L114/81.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus)
    

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. anti-Kv1.2 scFv [K14/16] with sortase tag

    Plasmid

    #204425

    Purpose

    Mammalian Expression of anti-Kv1.2 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K14/16.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting Kv1.2 (Homo sapiens)
    

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pExp-His-zBasic-RBD-Avi

    Plasmid

    #195000

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Article

    Fischer et al bioRxiv 2023.01.10.523494

    Insert

    RBD (S SARS-CoV-2)
    

    Tags

    Avi-tag and His8-Zbasic

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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